CAJ | 학술논문

目的：观察桂枝汤对4-甲基邻苯二酚(4-MC)诱导的心脏交感神经的抑制作用。方法10 mg · L-1的4-MC (10μg·kg-1体重)腹腔注射30d诱导建立心脏交感神经芽生模型,分别给予美托洛尔和不同桂芍比例的桂枝汤(桂芍比分别为1：1、1：2、2：1)进行药物干预。观察大鼠心率、心电图改变,ELISA法检测左心室和右心房心肌匀浆中去甲肾上腺素(NE)、生长相关蛋白(GAP-43)、酪氨酸羟化酶(TH)、乙酰胆碱酶( CHAT )的含量,免疫荧光法观察右心房、左心室中酪氨酸羟化酶( TH)阳性神经纤维的分布。结果4-MC造成心脏交感神经芽生,对CHAT无影响。与模型对照组比较,各药物组心率降低；各组心电图无明显差异；相比模型对照组,各药物组右心房和左心室中 NE、GAP-43、TH的含量降低(P<0.05)；免疫荧光示各药物组右心房和左心室中TH阳性神经分布少于模型对照组(P<0.05)。桂芍比为1：1和1：2时药效最佳,与美托洛尔相似。结论桂枝汤(桂芍比为1：1和1：2)可以有效减轻4-MC诱导的心脏交感神经芽生。
목적：관찰계지탕대4-갑기린분이분(4-MC)유도적심장교감신경적억제작용。방법10 mg · L-1적4-MC (10μg·kg-1체중)복강주사30d유도건립심장교감신경아생모형,분별급여미탁락이화불동계작비례적계지탕(계작비분별위1：1、1：2、2：1)진행약물간예。관찰대서심솔、심전도개변,ELISA법검측좌심실화우심방심기균장중거갑신상선소(NE)、생장상관단백(GAP-43)、락안산간화매(TH)、을선담감매( CHAT )적함량,면역형광법관찰우심방、좌심실중락안산간화매( TH)양성신경섬유적분포。결과4-MC조성심장교감신경아생,대CHAT무영향。여모형대조조비교,각약물조심솔강저；각조심전도무명현차이；상비모형대조조,각약물조우심방화좌심실중 NE、GAP-43、TH적함량강저(P<0.05)；면역형광시각약물조우심방화좌심실중TH양성신경분포소우모형대조조(P<0.05)。계작비위1：1화1：2시약효최가,여미탁락이상사。결론계지탕(계작비위1：1화1：2)가이유효감경4-MC유도적심장교감신경아생。
Aim To investigate the preventive effect of Guizhi decoction on cardiac sympathetic sprouting induced by 4-Methylcatechol (4-MC) . Methods The rat models of cardiac sympathetic sprouting were in-duced by 10 mg · L-1 4-MC ( 10μg · kg-1 body weight, i. p. ) . Guizhi decoctions ( with different pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba, 1 : 1, 1 : 2 and 2 : 1) and metoprolol were administered to the rats. Heart rate and electrocardio-gram ( ECG ) were observed, the content of norepi-nephrine (NE), growth associated protein (GAP-43), tyrosine hydroxylase ( TH ) and acetylcholine transfer-ase enzyme ( CHAT) in myocardial homogenate of left ventricular and right atrial were determined by ELISA method, and immunofluorescence assay was used to observe cardiac nerve sprouting and sympathetic distri-bution. Results 4-MC caused cardiac sympathetic sprouting and parasympathetic was not influenced. Heart rate of the model group was improved significant-ly and higher than that of the other groups . Compared with the model group, the content of NE, GAP-43 and TH in left ventricle and right atrium of the metoprolol group and Guizhi decoction group were decreased( P<0.05 ) , and the immunofluorescence result showed that the distribution of TH positive nerve was reduced sig-nificantly(P<0.05). It was demonstrated that Guizhi decoction of the proportion between Ramulus Cinnamo-mi and Radix Paeoniae Alba 1 : 1 and 1 : 2 had the best efficacy, which was similar to the efficacy of meto-prolol. Conclusion Guizhi decoction ( with the pro-portion between Ramulus Cinnamomi and Radix Paeon-iae Alba 1 : 1 and 1 : 2 ) effectively inhibits the cardi-ac sympathetic sprouting induced by 4-MC.