CAJ | 학술논문

目的：研究松果菊苷( ECH)对Aβ25-35引起的阿尔采末病( AD)大鼠的学习与记忆能力的改善和氧自由基水平的影响。方法60只SD大鼠,体质量(300依10) g,随机分为假手术组、模型组、ECH 高、中、低剂量组(40、20、10 mg ·kg-1·d-1)、阳性药石杉碱甲组(Hup-A,0.02 mg·kg-1·d-1)。水迷宫实验评价其学习记忆能力的改变。检测大脑皮质和海马中丙二醛( MDA)、一氧化碳( NO)的含量和超氧化物歧化酶( SOD)、一氧化氮合酶( NOS)的活性。结果 Aβ25-35能够严重损害大鼠学习记忆能力；与模型组大鼠相比,ECH不同剂量治疗组均能够减少其对学习记忆能力的损害(P<0.01, P<0.05)。 ECH治疗组大鼠大脑皮质和海马组织中的MDA含量明显下降,SOD的活性明显升高,明显减少NO、NOS的释放(P<0.01, P<0.05)。结论 ECH可以改善AD大鼠学习记忆力,其作用机制可能与加快氧自由基的清除能力和降低大鼠脑内氧化应激水平有关。
목적：연구송과국감( ECH)대Aβ25-35인기적아이채말병( AD)대서적학습여기억능력적개선화양자유기수평적영향。방법60지SD대서,체질량(300의10) g,수궤분위가수술조、모형조、ECH 고、중、저제량조(40、20、10 mg ·kg-1·d-1)、양성약석삼감갑조(Hup-A,0.02 mg·kg-1·d-1)。수미궁실험평개기학습기억능력적개변。검측대뇌피질화해마중병이철( MDA)、일양화탄( NO)적함량화초양화물기화매( SOD)、일양화담합매( NOS)적활성。결과 Aβ25-35능구엄중손해대서학습기억능력；여모형조대서상비,ECH불동제량치료조균능구감소기대학습기억능력적손해(P<0.01, P<0.05)。 ECH치료조대서대뇌피질화해마조직중적MDA함량명현하강,SOD적활성명현승고,명현감소NO、NOS적석방(P<0.01, P<0.05)。결론 ECH가이개선AD대서학습기억력,기작용궤제가능여가쾌양자유기적청제능력화강저대서뇌내양화응격수평유관。
Aim To investigate the effects of echina-coside ( ECH ) on the learning and memory capacities and brain level of oxygen free radicals of rats with de-mentia induced by amyloid β-peptide. Methods Six-ty Sprague Dawley rats, weighing (300±10) g, were randomly divided with 10 rats pergroup into 6 groups:sham operated group, model, ECH high dose (40 mg ·kg-1·d-1), ECH middle dose (20 mg· kg-1· d-1), ECH low dose (10 mg·kg-1·d-1) and Hup A (Huperzine A, 0. 02 mg·kg-1·d-1) group. Mor-ris maze tests were conducted for evaluating the learn-ing and memory ability. Content of malondialdehyde (MDA), nitric oxide (NO) and activities of superox-ide dismutase ( SOD) and NO synthase ( NOS) in the hippocampus and cortex were detected. ResultsAβ25-35 induced significant learning and memory im-pairment in the rats. Compared with the rats in model group, those treated with ECH at different doses all manifested alleviation of learning and memory impair-ment ( P<0 . 01 , P<0 . 05 ) . Cotents of MDA of ECH treatment group were obviously decreased, while SOD activities were obviously increased, and significantly reduced the release of NO and NOS in the hippocam-pus and cortex brain tissue ( P <0 . 01 , P <0.05 ) . Conclusion ECH can enhance the learning and mem-ory ability in rats with AD, which is presumably relat-ed to accelerating the cleaning of oxygen free radicals and reducing oxidative stress in brain of AD rats.