中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
9期
1302-1305
,共4页
丁慧%陈虹%姜勇%屠鹏飞%马婧怡%张万鑫
丁慧%陳虹%薑勇%屠鵬飛%馬婧怡%張萬鑫
정혜%진홍%강용%도붕비%마청이%장만흠
松果菊苷%阿尔采末病%水迷宫%Aβ25-35%氧自由基%海马组织%大脑皮层
鬆果菊苷%阿爾採末病%水迷宮%Aβ25-35%氧自由基%海馬組織%大腦皮層
송과국감%아이채말병%수미궁%Aβ25-35%양자유기%해마조직%대뇌피층
echinacoside%Alzheimer's disease%Mor-ris water maze%amyloid β-protein fragment 25 -35%oxygen free radicals%hippocampus%cerebral cortex
目的:研究松果菊苷( ECH)对Aβ25-35引起的阿尔采末病( AD)大鼠的学习与记忆能力的改善和氧自由基水平的影响。方法60只SD大鼠,体质量(300依10) g,随机分为假手术组、模型组、ECH 高、中、低剂量组(40、20、10 mg ·kg-1·d-1)、阳性药石杉碱甲组(Hup-A,0.02 mg·kg-1·d-1)。水迷宫实验评价其学习记忆能力的改变。检测大脑皮质和海马中丙二醛( MDA)、一氧化碳( NO)的含量和超氧化物歧化酶( SOD)、一氧化氮合酶( NOS)的活性。结果 Aβ25-35能够严重损害大鼠学习记忆能力;与模型组大鼠相比,ECH不同剂量治疗组均能够减少其对学习记忆能力的损害(P<0.01, P<0.05)。 ECH治疗组大鼠大脑皮质和海马组织中的MDA含量明显下降,SOD的活性明显升高,明显减少NO、NOS的释放(P<0.01, P<0.05)。结论 ECH可以改善AD大鼠学习记忆力,其作用机制可能与加快氧自由基的清除能力和降低大鼠脑内氧化应激水平有关。
目的:研究鬆果菊苷( ECH)對Aβ25-35引起的阿爾採末病( AD)大鼠的學習與記憶能力的改善和氧自由基水平的影響。方法60隻SD大鼠,體質量(300依10) g,隨機分為假手術組、模型組、ECH 高、中、低劑量組(40、20、10 mg ·kg-1·d-1)、暘性藥石杉堿甲組(Hup-A,0.02 mg·kg-1·d-1)。水迷宮實驗評價其學習記憶能力的改變。檢測大腦皮質和海馬中丙二醛( MDA)、一氧化碳( NO)的含量和超氧化物歧化酶( SOD)、一氧化氮閤酶( NOS)的活性。結果 Aβ25-35能夠嚴重損害大鼠學習記憶能力;與模型組大鼠相比,ECH不同劑量治療組均能夠減少其對學習記憶能力的損害(P<0.01, P<0.05)。 ECH治療組大鼠大腦皮質和海馬組織中的MDA含量明顯下降,SOD的活性明顯升高,明顯減少NO、NOS的釋放(P<0.01, P<0.05)。結論 ECH可以改善AD大鼠學習記憶力,其作用機製可能與加快氧自由基的清除能力和降低大鼠腦內氧化應激水平有關。
목적:연구송과국감( ECH)대Aβ25-35인기적아이채말병( AD)대서적학습여기억능력적개선화양자유기수평적영향。방법60지SD대서,체질량(300의10) g,수궤분위가수술조、모형조、ECH 고、중、저제량조(40、20、10 mg ·kg-1·d-1)、양성약석삼감갑조(Hup-A,0.02 mg·kg-1·d-1)。수미궁실험평개기학습기억능력적개변。검측대뇌피질화해마중병이철( MDA)、일양화탄( NO)적함량화초양화물기화매( SOD)、일양화담합매( NOS)적활성。결과 Aβ25-35능구엄중손해대서학습기억능력;여모형조대서상비,ECH불동제량치료조균능구감소기대학습기억능력적손해(P<0.01, P<0.05)。 ECH치료조대서대뇌피질화해마조직중적MDA함량명현하강,SOD적활성명현승고,명현감소NO、NOS적석방(P<0.01, P<0.05)。결론 ECH가이개선AD대서학습기억력,기작용궤제가능여가쾌양자유기적청제능력화강저대서뇌내양화응격수평유관。
Aim To investigate the effects of echina-coside ( ECH ) on the learning and memory capacities and brain level of oxygen free radicals of rats with de-mentia induced by amyloid β-peptide. Methods Six-ty Sprague Dawley rats, weighing (300±10) g, were randomly divided with 10 rats pergroup into 6 groups:sham operated group, model, ECH high dose (40 mg ·kg-1·d-1), ECH middle dose (20 mg· kg-1· d-1), ECH low dose (10 mg·kg-1·d-1) and Hup A (Huperzine A, 0. 02 mg·kg-1·d-1) group. Mor-ris maze tests were conducted for evaluating the learn-ing and memory ability. Content of malondialdehyde (MDA), nitric oxide (NO) and activities of superox-ide dismutase ( SOD) and NO synthase ( NOS) in the hippocampus and cortex were detected. ResultsAβ25-35 induced significant learning and memory im-pairment in the rats. Compared with the rats in model group, those treated with ECH at different doses all manifested alleviation of learning and memory impair-ment ( P<0 . 01 , P<0 . 05 ) . Cotents of MDA of ECH treatment group were obviously decreased, while SOD activities were obviously increased, and significantly reduced the release of NO and NOS in the hippocam-pus and cortex brain tissue ( P <0 . 01 , P <0.05 ) . Conclusion ECH can enhance the learning and mem-ory ability in rats with AD, which is presumably relat-ed to accelerating the cleaning of oxygen free radicals and reducing oxidative stress in brain of AD rats.