CAJ | 학술논문

目的：探讨D-半乳糖是否会引起骨质疏松,与去卵巢致骨质疏松有何异同,去卵巢后再加上D-半乳糖是否会加速加重骨质疏松的进展,补充雌激素是否有预防作用。方法 SPF级小鼠60只,随机分为假手术组、D-半乳糖组、去卵巢组、去卵巢+D-半乳糖组、去卵巢+D-半乳糖+己烯雌酚组和D-半乳糖+己烯雌酚组。实验70 d后,取右胫骨进行不脱钙骨组织切片及骨形态学计量学观察与评价。结果与假手术组比较,去卵巢组%Tb. Ar、Tb. Th、Tb. N明显降低(P<0.01),Tb. Sp明显升高(P<0.05),反映骨吸收指标的Oc. Pm、Oc. No.、%Oc. S、Oc. N/mm明显升高(P<0.01),反映骨形成指标的%L. Pm、MAR、BFR/TV、BFR/BV、BFR/BS明显降低( P<0.01)。 D-半乳糖组反映骨吸收的4个指标明显升高(P<0.05),反映骨形成的4个指标明显降低(P<0.05)。去卵巢+D-半乳糖组也有明显的骨质疏松的表现,但各指标与去卵巢组相比,差异没有显著性,与D-半乳糖组相比,各指标差异也没有显著性。雌激素对D-半乳糖和去卵巢联合D-半乳糖导致的骨质疏松相关指标均有明显的对抗作用。结论去卵巢、D-半乳糖、去卵巢联合D-半乳糖均能建立小鼠骨质疏松模型,雌激素能有效防止D-半乳糖和去卵巢联合D-半乳糖致骨量丢失。
목적：탐토D-반유당시부회인기골질소송,여거란소치골질소송유하이동,거란소후재가상D-반유당시부회가속가중골질소송적진전,보충자격소시부유예방작용。방법 SPF급소서60지,수궤분위가수술조、D-반유당조、거란소조、거란소+D-반유당조、거란소+D-반유당+기희자분조화D-반유당+기희자분조。실험70 d후,취우경골진행불탈개골조직절편급골형태학계량학관찰여평개。결과여가수술조비교,거란소조%Tb. Ar、Tb. Th、Tb. N명현강저(P<0.01),Tb. Sp명현승고(P<0.05),반영골흡수지표적Oc. Pm、Oc. No.、%Oc. S、Oc. N/mm명현승고(P<0.01),반영골형성지표적%L. Pm、MAR、BFR/TV、BFR/BV、BFR/BS명현강저( P<0.01)。 D-반유당조반영골흡수적4개지표명현승고(P<0.05),반영골형성적4개지표명현강저(P<0.05)。거란소+D-반유당조야유명현적골질소송적표현,단각지표여거란소조상비,차이몰유현저성,여D-반유당조상비,각지표차이야몰유현저성。자격소대D-반유당화거란소연합D-반유당도치적골질소송상관지표균유명현적대항작용。결론거란소、D-반유당、거란소연합D-반유당균능건립소서골질소송모형,자격소능유효방지D-반유당화거란소연합D-반유당치골량주실。
Aim To investigate whether D-galactose cause osteoporosis and the difference compared with the osteoporosis induced by ovariectomy, and to deter-mine whether ovariectomy coupled with D-galactose ac-celerated the progress of osteoporosis and whether es-trogen had a preventive effect on these osteoporosis models. Methods Sixty SPF mice were randomly divided into six groups , namely sham-operated group, D-galactose group, OVX group, OVX + D-galactose group, OVX + D-galactose + diethylstilbestrol group and D-galactose + diethylstilbestrol group. Seventy days later, the right tibia was processed with undecal-cified sections for bone histomorphometric analysis. Results Compared with the sham-operated group, %Tb. Ar, Tb. Th and Tb. N decrease by 50. 4%, 25. 4%, 50. 9% ( P <0.01 ) respectively, Tb. Sp in-creased by 169. 4% (P <0.05), Oc. pm, Oc. No. ,%Oc. S, Oc. N/mm which reflected bone absorption significantly increased ( P < 0.01 ) , and % L. Pm, MAR, BFR/TV, BFR/BV, BFR/BS which reflected bone formation significantly decreased ( P <0.01 ) in OVX group. %Tb. Ar decreased by 30. 4% in D-ga-lactose group, but there was no statistically significant difference. However, the four parameters reflected the bone absorption in D-galactose group increased signifi-cantly ( P<0.05 ) , while the four parameters reflected bone formation decreased significantly ( P < 0.05 ) . OVX+D-galactose group has obvious performance of osteoporosis, but there was no significant difference compared to OVX group, nor to D-galactose group. Estrogen had significant preventive effect on related pa-rameters of osteoporosis induced by D-galactose and o-variectomy coupled with D-galactose. ConclusionsOsteoporosis model of mice can be established by OVX, D-galactose and OVX +D-galactose. Estrogen can effectively prevent bone loss induced by D-galac-tose and OVX+ D-galactose.