中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
9期
1276-1280
,共5页
张秀丽%闫巍巍%刘东志%张励才
張秀麗%閆巍巍%劉東誌%張勵纔
장수려%염외외%류동지%장려재
ATP敏感性钾离子通道%下丘脑%室旁核%完全弗氏佐剂%炎性痛%二氮嗪
ATP敏感性鉀離子通道%下丘腦%室徬覈%完全弗氏佐劑%炎性痛%二氮嗪
ATP민감성갑리자통도%하구뇌%실방핵%완전불씨좌제%염성통%이담진
ATP-sensitive potassium channel%hypo-thalamic%paraventricular nucleus%complete freund's adjuvant%inflammatory pain%diaoxide
目的：探讨下丘脑室旁核 ATP 敏感性钾离子通道( KATP通道)在大鼠炎性痛中的作用。方法裔 SD 大鼠(250~280 g),采用随机数字法分为5组(每组6只)：正常组( Normal组)、完全弗氏佐剂致炎性痛组( CFA组)、生理盐水组( Saline组)、KATP通道特异性激动剂组( Diaoxide组)和激动剂溶媒组( Vehicle组)。以热痛敏刺激仪检测各组大鼠热缩足潜伏期( TWL),观察痛行为学变化；以免疫荧光技术观察室旁核KATP通道和脊髓背角c-Fos阳性细胞数的变化。并观察KATP通道激动剂对大鼠痛行为和脊髓背角c-Fos表达的影响。结果①与术前和Saline组相比,CFA组大鼠炎症侧后足术后d 1、d 3和d 7的TWL降低(P<0.05),CFA组术后d 3、d 7的KATP阳性细胞数减少(P<0.01),脊髓背角c-Fos阳性细胞数增加( P<0.01)；②与Vehicle组相比,激动剂组大鼠热痛觉过敏减轻( P<0.01),脊髓背角c-Fos阳性细胞数减少(P<0.01)。结论下丘脑室旁核KATP通道可能与CFA所致炎性痛的发生机制相关。
目的：探討下丘腦室徬覈 ATP 敏感性鉀離子通道( KATP通道)在大鼠炎性痛中的作用。方法裔 SD 大鼠(250~280 g),採用隨機數字法分為5組(每組6隻)：正常組( Normal組)、完全弗氏佐劑緻炎性痛組( CFA組)、生理鹽水組( Saline組)、KATP通道特異性激動劑組( Diaoxide組)和激動劑溶媒組( Vehicle組)。以熱痛敏刺激儀檢測各組大鼠熱縮足潛伏期( TWL),觀察痛行為學變化；以免疫熒光技術觀察室徬覈KATP通道和脊髓揹角c-Fos暘性細胞數的變化。併觀察KATP通道激動劑對大鼠痛行為和脊髓揹角c-Fos錶達的影響。結果①與術前和Saline組相比,CFA組大鼠炎癥側後足術後d 1、d 3和d 7的TWL降低(P<0.05),CFA組術後d 3、d 7的KATP暘性細胞數減少(P<0.01),脊髓揹角c-Fos暘性細胞數增加( P<0.01)；②與Vehicle組相比,激動劑組大鼠熱痛覺過敏減輕( P<0.01),脊髓揹角c-Fos暘性細胞數減少(P<0.01)。結論下丘腦室徬覈KATP通道可能與CFA所緻炎性痛的髮生機製相關。
목적：탐토하구뇌실방핵 ATP 민감성갑리자통도( KATP통도)재대서염성통중적작용。방법예 SD 대서(250~280 g),채용수궤수자법분위5조(매조6지)：정상조( Normal조)、완전불씨좌제치염성통조( CFA조)、생리염수조( Saline조)、KATP통도특이성격동제조( Diaoxide조)화격동제용매조( Vehicle조)。이열통민자격의검측각조대서열축족잠복기( TWL),관찰통행위학변화；이면역형광기술관찰실방핵KATP통도화척수배각c-Fos양성세포수적변화。병관찰KATP통도격동제대대서통행위화척수배각c-Fos표체적영향。결과①여술전화Saline조상비,CFA조대서염증측후족술후d 1、d 3화d 7적TWL강저(P<0.05),CFA조술후d 3、d 7적KATP양성세포수감소(P<0.01),척수배각c-Fos양성세포수증가( P<0.01)；②여Vehicle조상비,격동제조대서열통각과민감경( P<0.01),척수배각c-Fos양성세포수감소(P<0.01)。결론하구뇌실방핵KATP통도가능여CFA소치염성통적발생궤제상관。
Aim To explore the role of KATP in the pa-raventricular nucleus in inflammatory pain. MethodsMale Sprague-Dawley rats,250~280 g, were randomly assigned into 5 groups ( each, n =6 ): Normal group, Saline group ( for control, subcutaneous injection of 100 μl of saline into the plantar surface of the left hindpaw) , CFA group ( subcutaneous injection of 100μl of complete freund's adjuvant into the plantar sur-face of the left hindpaw) , Vehicle group ( treated with dimethylsulfoxide), KATP selective agonist group(trea-ted with diaoxide) . Rats in each group were tested for TWL with radiant heat apparatus. Immunofluorescent technique was used to label KATP in PVN and c-Fos in lumber spinal cord. Three days after injected with CFA, a selective KATP agonist, diaoxide, was injected into one side of PVN to test its effect on inflammatory pain and c-Fos expression in lumber spinal cord. Re-sults ① Compared with pre-operation and saline group, rats showed significantly lower TWL on day 1, 3, 7 after injection of CFA;the numbers of KATP posi-tive cells were significantly lower; the numbers of c-Fos positive cells were significantly higher. ② Com-pared with those of vehicle group, TWL and the num-ber of c-Fos in lumber spinal cord were both signifi-cantly lower after injection of diaoxide into one side of PVN. Conclusion KATP in PVN is related to inflam-matory pain.
