CAJ | 학술논문

目的：研究水杨酸钠对大鼠海马神经元上电压门控性钙离子通道的作用，为海马参与耳鸣引起的情绪变化提供电生理学的依据。方法利用脑片膜片钳技术观察水杨酸钠对大鼠海马神经元电压门控性钙离子通道电生理学特性的影响。结果水杨酸钠对电压门控性钙离子通道电流( ICa )有抑制作用，而且具有浓度依赖性(0.1~10 mmol · L-1)。水杨酸钠对海马神经元ICa的半抑制浓度( IC50)为1.64。1 mmol·L-1水杨酸钠对ICa的稳态激活动力学有明显影响，使ICa的稳态激活曲线向超极化方向移动大约9 mV左右，但是对其稳态失活动力学没有明显影响。结论水杨酸钠以浓度依赖的方式抑制 ICa ，对其稳态激活动力学有明显影响。水杨酸钠对大鼠海马神经元上ICa的影响可能与海马参与耳鸣引起的情绪变化相关。
목적：연구수양산납대대서해마신경원상전압문공성개리자통도적작용，위해마삼여이명인기적정서변화제공전생이학적의거。방법이용뇌편막편겸기술관찰수양산납대대서해마신경원전압문공성개리자통도전생이학특성적영향。결과수양산납대전압문공성개리자통도전류( ICa )유억제작용，이차구유농도의뢰성(0.1~10 mmol · L-1)。수양산납대해마신경원ICa적반억제농도( IC50)위1.64。1 mmol·L-1수양산납대ICa적은태격활동역학유명현영향，사ICa적은태격활곡선향초겁화방향이동대약9 mV좌우，단시대기은태실활동역학몰유명현영향。결론수양산납이농도의뢰적방식억제 ICa ，대기은태격활동역학유명현영향。수양산납대대서해마신경원상ICa적영향가능여해마삼여이명인기적정서변화상관。
Aim To provide an electrophysiological basis for hippocampus involved emotional changes caused by tinnitus, the effects of salicylate on electro-physiological characteristics of the voltage-gated calci-um channels in hippocampal neurons were performed. Method The effects of salicylate on voltage-gated cal-cium channels in rat hippocampal neurons were stud-ied, using the whole-cell voltage clamp method. Re-sults Salicylate blocked the voltage-gated calcium channels ( ICa ) in a concentration-dependent manner (0.1~10 mmol · L-1 ) . The half-inhibition concen-tration ( IC50 ) values for the blocking action of salicy-late on ICa were 1.64 mmol·L-1 . With 1 mmol·L-1 salicylate applicalted into bath solution, the steady-state activation curve of calcium channel was shifted by about 9 mV in the hyperpolarizing direction, and its steady-state inactivation curve was not changed. Con-clusion Salicylate inhibits ICa in rat hippocampal neu-rons and significantly affects the activation kinetics of ICa ,which could be related to emotional changes caused by tinnitus.