中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2014年
9期
1225-1228,1229
,共5页
马开利%宋连昆%苑玉和%张莹%李莉%杨金玲%朱平%陈乃宏
馬開利%宋連昆%苑玉和%張瑩%李莉%楊金玲%硃平%陳迺宏
마개리%송련곤%원옥화%장형%리리%양금령%주평%진내굉
α突触核蛋白%核苷酸序列%圆二色谱%GC-box样序列%核定位%帕金森病
α突觸覈蛋白%覈苷痠序列%圓二色譜%GC-box樣序列%覈定位%帕金森病
α돌촉핵단백%핵감산서렬%원이색보%GC-box양서렬%핵정위%파금삼병
α-synuclein%nucleotide sequences%circu-lar dichroism%GC-box like sequence%nuclear localiza-tion%Parkinson's disease
目的：体外筛选α突触核蛋白可能作用的核苷酸序列。方法根据碱基组成特征合成一系列的核苷酸序列,与α突触核蛋白-绿色荧光蛋白(α-synuclein-green fluorescent protein,α-syn-GFP)融合蛋白混合,采用圆二色谱分析色谱峰的变化情况。结果α突触核蛋白-绿色荧光蛋白融合蛋白混合入GC-box样核苷酸序列后导致色谱峰发生较大变化。结论α突触核蛋白转位进入细胞核后,可能通过作用于GC box样核苷酸序列,参与细胞核内的基因表达调控,从而发挥其生理或病理作用。
目的：體外篩選α突觸覈蛋白可能作用的覈苷痠序列。方法根據堿基組成特徵閤成一繫列的覈苷痠序列,與α突觸覈蛋白-綠色熒光蛋白(α-synuclein-green fluorescent protein,α-syn-GFP)融閤蛋白混閤,採用圓二色譜分析色譜峰的變化情況。結果α突觸覈蛋白-綠色熒光蛋白融閤蛋白混閤入GC-box樣覈苷痠序列後導緻色譜峰髮生較大變化。結論α突觸覈蛋白轉位進入細胞覈後,可能通過作用于GC box樣覈苷痠序列,參與細胞覈內的基因錶達調控,從而髮揮其生理或病理作用。
목적：체외사선α돌촉핵단백가능작용적핵감산서렬。방법근거감기조성특정합성일계렬적핵감산서렬,여α돌촉핵단백-록색형광단백(α-synuclein-green fluorescent protein,α-syn-GFP)융합단백혼합,채용원이색보분석색보봉적변화정황。결과α돌촉핵단백-록색형광단백융합단백혼합입GC-box양핵감산서렬후도치색보봉발생교대변화。결론α돌촉핵단백전위진입세포핵후,가능통과작용우GC box양핵감산서렬,삼여세포핵내적기인표체조공,종이발휘기생리혹병리작용。
Aim To study the screening of the nucleo-tide sequences might be affected by α-syn in vitro. Methods The nucleotide sequences were synthesized according to the feature of base composition, and then mixed with the α-syn-GFP. The CD was used to ana-lyse the changes of the peak. Result The peak of the CD changed greatly when the α-syn-GFP mixed with the GC-box like sequence. Conclusion The α-syn-GFP might affect the GC-box like sequence after trans-located into the nuclei. Then, it plays a role in physio-logical and pathological conditions by affecting the reg-ulation of gene expression.
