CAJ | 학술논문

传统虾夷扇贝外套膜直接水解后产物风味及安全问题突出，通过等电点絮凝法脱除虾夷扇贝外套膜中的砂分、色素、腥味及脂肪等杂质，分离回收得到外套膜蛋白。以外套膜分离蛋白为底物，利用Protamex复合蛋白酶和Flavourzyme风味蛋白酶对所得到的分离蛋白进行水解。通过单因素试验和正交试验研究了酶用量、水解温度、水解时间以及pH值对水解度的影响，并确定Protamex复合蛋白酶水解外套膜分离蛋白最佳工艺条件为酶用量0．075AU/g ，水解时间5h，pH7．0，水解温度55℃。利用优化条件水解分离蛋白，所得酶解液再经浓缩冻干后进行微生物检验和重金属检验，微生物检测结果表明，微生物总数为280个/g ，大肠杆菌群则未检出，重金属分析表明，与原料外套膜相比，分离处理能够有效降低重金属含量。因此，基于外套膜分离蛋白，再进行酶解处理，有效的解决了传统水解方式带来的食品风味问题以及食品安全问题。为虾夷扇贝外套膜的高值化利用提供新的思路。
전통하이선패외투막직접수해후산물풍미급안전문제돌출，통과등전점서응법탈제하이선패외투막중적사분、색소、성미급지방등잡질，분리회수득도외투막단백。이외투막분리단백위저물，이용Protamex복합단백매화Flavourzyme풍미단백매대소득도적분리단백진행수해。통과단인소시험화정교시험연구료매용량、수해온도、수해시간이급pH치대수해도적영향，병학정Protamex복합단백매수해외투막분리단백최가공예조건위매용량0．075AU/g ，수해시간5h，pH7．0，수해온도55℃。이용우화조건수해분리단백，소득매해액재경농축동간후진행미생물검험화중금속검험，미생물검측결과표명，미생물총수위280개/g ，대장간균군칙미검출，중금속분석표명，여원료외투막상비，분리처리능구유효강저중금속함량。인차，기우외투막분리단백，재진행매해처리，유효적해결료전통수해방식대래적식품풍미문제이급식품안전문제。위하이선패외투막적고치화이용제공신적사로。
T raditional production of protein hydrolysates from scallop mantle directly is involved in out-standing flavor and safety issues .In this paper ,protein isolates were collected from mantle of yesso scallop Patinopecten yessoensis by pH shift processing and Protamex and Flavourzyme hydrolysis .The orthogonal test revealed that the hydrolysis was affected by enzyme addition level ,temperature ,time and pH .The optimal Protamex hydrolysis process was shown to be carried out under conditions of enzyme addition at a dose of 0 .075AU/g ,5h ,pH 7 .0 ,and 55 ℃ .The microorganism species composition and density and heavy metal levels were detected in the hydrolyzed protein isolates freeze-dried .Results showed that total colony counts were 280 cfu/g ,and that Bacillus coil was not detected .It was found that heavy metals were to some extent removed in the protein hydrolysates compared with the raw mantles .The findings in-dicate that the mantle protein isolates by the enzymatic process lead to an effective solution to the food fla-vors and food safety and provide a new way to make a full use of the scallop mantles .