水产科学
水產科學
수산과학
FISHERIES SCIENCE
2014年
9期
562-568
,共7页
李建光%徐永平%李晓宇%王丽丽%包鹏云%蒲红宇%车鉴%王熙涛
李建光%徐永平%李曉宇%王麗麗%包鵬雲%蒲紅宇%車鑒%王熙濤
리건광%서영평%리효우%왕려려%포붕운%포홍우%차감%왕희도
变性梯度凝胶电泳%菌群结构%刺参
變性梯度凝膠電泳%菌群結構%刺參
변성제도응효전영%균군결구%자삼
denaturing gradient gel electrophoresis%bacterial community%Apostichopus japonicas
应用变性梯度凝胶电泳技术分析在不同养殖季节环境中,仿刺参肠道、养殖水体和池塘底泥中菌群结构的特点。试验结果表明,在不同的养殖季节中,仿刺参肠道、养殖水体和池塘底泥中菌群结构仅有很少差别,仿刺参菌群组成相对稳定。γ-变形杆菌和α-变形杆菌的菌属是出现最多的菌群,其次是β-变形杆菌、ε-变形杆菌、螺旋体目、拟杆菌门、纤维黏菌目和衣原体目。刺参肠道的优势菌属主要由弧菌属、玫瑰杆菌属、发光杆菌属和假交替单胞菌属等组成;养殖水体的优势菌属主要由弧菌属、玫瑰杆菌属、绿屈挠菌属和希瓦氏菌属等组成;池塘底泥的优势菌属主要由弧菌属、土壤杆菌属和小球菌属等组成。
應用變性梯度凝膠電泳技術分析在不同養殖季節環境中,倣刺參腸道、養殖水體和池塘底泥中菌群結構的特點。試驗結果錶明,在不同的養殖季節中,倣刺參腸道、養殖水體和池塘底泥中菌群結構僅有很少差彆,倣刺參菌群組成相對穩定。γ-變形桿菌和α-變形桿菌的菌屬是齣現最多的菌群,其次是β-變形桿菌、ε-變形桿菌、螺鏇體目、擬桿菌門、纖維黏菌目和衣原體目。刺參腸道的優勢菌屬主要由弧菌屬、玫瑰桿菌屬、髮光桿菌屬和假交替單胞菌屬等組成;養殖水體的優勢菌屬主要由弧菌屬、玫瑰桿菌屬、綠屈撓菌屬和希瓦氏菌屬等組成;池塘底泥的優勢菌屬主要由弧菌屬、土壤桿菌屬和小毬菌屬等組成。
응용변성제도응효전영기술분석재불동양식계절배경중,방자삼장도、양식수체화지당저니중균군결구적특점。시험결과표명,재불동적양식계절중,방자삼장도、양식수체화지당저니중균군결구부유흔소차별,방자삼균군조성상대은정。γ-변형간균화α-변형간균적균속시출현최다적균군,기차시β-변형간균、ε-변형간균、라선체목、의간균문、섬유점균목화의원체목。자삼장도적우세균속주요유호균속、매괴간균속、발광간균속화가교체단포균속등조성;양식수체적우세균속주요유호균속、매괴간균속、록굴뇨균속화희와씨균속등조성;지당저니적우세균속주요유호균속、토양간균속화소구균속등조성。
The aim of the study was to use a combination of denaturing gradient gel electrophoresis tech-nology to evaluate the bacterial communities of artificial diet-fed sea cucumber (Apostichopus japonicus) adults in different culture seasons .Overall ,the results showed that bacterial communities associated with sea cucumbers were considerable stable in different culture seasons ,little differences in the bacterial as-semblages .Phylogenetic analysis demonstrated that the most frequently observed species were γ-Pro-teobacteria and α-Proteobacteria ,additional species were β-Proteobacteria ,ε-Proteobacteria ,Spirochaet-ales ,Bacteroidetes ,Cytophagales and Chlamydiales groups .The dominant bacterial community composi-tion including V ibrio sp ., Roseobacter sp ., Photobacterium sp . in intestine samples ;V ibrio sp ., Ro-seobacter sp .,Cytophage sp .was the dominant group in the seawater samples ;while Vibrio sp .,Micro-coccus sp .,Pseudoalteromonas sp .,Photobacterium sp .,Chloroflexi sp .,Shewnella sp .,Pantoea sp ., X anthomonas sp .and ,A grobacterium sp .were the dominant group in the sediment samples .These find-ings provide the sequence foundation for the development of molecular assays that target sea cucumber bac-teria and probiotics .