CAJ | 학술논문

研究了地黄和深海鱼源活性小肽对鲤鱼生长和免疫功能的调节作用，探讨其作为鱼类饲料添加剂的可行性。试验一选取540尾平均体质量为（7．46±1．06） g的鲤鱼，随机分为9组饲喂基础日粮及添加了2％生地黄粉，4％生地黄粉，2％熟地黄粉，4％熟地黄粉，0．5％生地黄水提物，1％生地黄水提物，0．5％熟地黄水提物，1％熟地黄水提物的饲料。试验二选取360尾平均体质量为（5．47±0．3）g的鲤鱼，随机分为4组饲喂基础日粮和添加5％、10％和15％小肽的饲料。饲养80 d后，测定鲤鱼的生长指标和抗病性能，并检测鲤鱼脾脏IL-8的mRNA表达情况。结果显示，各种地黄制剂和不同剂量的小肽均可提高鲤鱼脾脏IL-8的表达，其中试验一的2％熟地黄粉、4％熟地黄粉、1％生地黄水提物、0．5％熟地黄水提物组和试验二的5％、10％小肽组与对照组均有显著性差异。地黄还可显著促进鲤的生长，提高其抗病力。因此，地黄和深海鱼源活性小肽均有望成为具有免疫调节作用的鱼类饲料添加剂。
연구료지황화심해어원활성소태대리어생장화면역공능적조절작용，탐토기작위어류사료첨가제적가행성。시험일선취540미평균체질량위（7．46±1．06） g적리어，수궤분위9조사위기출일량급첨가료2％생지황분，4％생지황분，2％숙지황분，4％숙지황분，0．5％생지황수제물，1％생지황수제물，0．5％숙지황수제물，1％숙지황수제물적사료。시험이선취360미평균체질량위（5．47±0．3）g적리어，수궤분위4조사위기출일량화첨가5％、10％화15％소태적사료。사양80 d후，측정리어적생장지표화항병성능，병검측리어비장IL-8적mRNA표체정황。결과현시，각충지황제제화불동제량적소태균가제고리어비장IL-8적표체，기중시험일적2％숙지황분、4％숙지황분、1％생지황수제물、0．5％숙지황수제물조화시험이적5％、10％소태조여대조조균유현저성차이。지황환가현저촉진리적생장，제고기항병력。인차，지황화심해어원활성소태균유망성위구유면역조절작용적어류사료첨가제。
The effects of Rehmannia glutinosa and marine source bioactive small peptide on growth and im-mune function in common carp (Cyprinus carpio) were investigated to develop Rehmannia glutinosa and the small peptide as fish feed additives .In Experiment 1 ,540 common carp juveniles with body weight of (7 .46 ± 1 .06) g were randomly allocated into nine groups ,and fed basal diet (control group) ,the diets containing radix rehmanniae pow der at a dose of 2% and 4% ,the diets containing prepared rehmannia root powder at a dose of 2% and 4% ,the diets containing radix rehmanniae extract at a rate of 0 .5% ,and 1% , or the diets containing prepared rehmannia root extract at a dose of 0 .5% and 1% for 80 days .In Experi-ment 2 ,360 common carp juveniles with body weight of (5 .47 ± 0 .3) g were randomly allocated into four groups ,and fed the basal diet (control group ) ,and the diets containing small peptide at a rate of 5% , 10% and 15% for 80 days .At the end of the experiments ,the growth performance ,disease resistance and the IL-8(interleukin-8) mRNA expression in spleen were studied in the juveniles .The results showed that the higher expression of IL-8 in the spleen was found in the juveniles fed the diets containing all sorts of re-hmannia preparations and different doses of the small peptide ,significantly higher in 2% prepared rehman-nia root powder ,4% prepared rehmannia root powder ,1% radix rehmanniae extract ,0 .5% prepared reh-mannia root extract and 5% and 10% small peptide groups than those in the control groups ,indicating that Rehmannia glutinosa can significantly promote the growth and disease resistance of common carp .Therefore ,Re-hmannia glutinosa and marine source bioactive peptide are expected to become good fish feed additives .