CAJ | 학술논문

在15℃下，将体质量（101．53±16．48） g的日本蟳暴露于对照及低质量浓度组（0．0025、0．005 mg/L ）、中质量浓度组（0．05、0．1 mg/L ）和高质量浓度组（0．25、0．5 mg/L ）的氯化镉中，于处理0、1、3、5、7、10、15 d和30 d时取血，测定其血清超氧化物歧化酶和过氧化氢酶活力、丙二醛含量；并于胁迫第30 d ，测定肝胰腺、肌肉、卵巢、鳃中的镉含量，以研究镉胁迫对日本蟳抗氧化能力及组织蓄积的影响。结果表明：在镉胁迫下，日本蟳超氧化物歧化酶和过氧化氢酶活力均呈先升后降趋势。中质量浓度组及高质量浓度组的镉胁迫对抗氧化酶活力诱导较快，而低质量浓度组诱导慢。胁迫第15 d和30 d ，除0．0025 m g/L组外，试验组两种酶活力均显著低于对照组，与镉胁迫质量浓度呈显著负相关（ P＜0．05）。0．0025 m g/L组丙二醛含量无明显变化，其他各组呈两种变化趋势：0．005、0．05 m g/L和0．1 mg/L组丙二醛含量先升后降再升，而高质量浓度（0．25、0．5 mg/L ）组丙二醛含量持续上升。至胁迫第10 d ，除0．0025 m g/L组外，试验组丙二醛含量均显著高于对照组，与镉胁迫质量浓度呈显著正相关。镉在日本蟳肝胰腺和鳃内的积累较多，而卵和肌肉仅在高质量浓度镉胁迫下才有微量的蓄积。各组肝胰腺中镉含量与质量浓度呈明显的质量浓度-效应关系。中质量浓度组和高质量浓度组鳃中镉含量显著增加，超过同一质量浓度组肝胰腺的镉蓄积量。
재15℃하，장체질량（101．53±16．48） g적일본심폭로우대조급저질량농도조（0．0025、0．005 mg/L ）、중질량농도조（0．05、0．1 mg/L ）화고질량농도조（0．25、0．5 mg/L ）적록화력중，우처리0、1、3、5、7、10、15 d화30 d시취혈，측정기혈청초양화물기화매화과양화경매활력、병이철함량；병우협박제30 d ，측정간이선、기육、란소、새중적력함량，이연구력협박대일본심항양화능력급조직축적적영향。결과표명：재력협박하，일본심초양화물기화매화과양화경매활력균정선승후강추세。중질량농도조급고질량농도조적력협박대항양화매활력유도교쾌，이저질량농도조유도만。협박제15 d화30 d ，제0．0025 m g/L조외，시험조량충매활력균현저저우대조조，여력협박질량농도정현저부상관（ P＜0．05）。0．0025 m g/L조병이철함량무명현변화，기타각조정량충변화추세：0．005、0．05 m g/L화0．1 mg/L조병이철함량선승후강재승，이고질량농도（0．25、0．5 mg/L ）조병이철함량지속상승。지협박제10 d ，제0．0025 m g/L조외，시험조병이철함량균현저고우대조조，여력협박질량농도정현저정상관。력재일본심간이선화새내적적루교다，이란화기육부재고질량농도력협박하재유미량적축적。각조간이선중력함량여질량농도정명현적질량농도-효응관계。중질량농도조화고질량농도조새중력함량현저증가，초과동일질량농도조간이선적력축적량。
The activities of superoxide dismutase (SOD) and catalase (CAT) ,and content of malonalde-hyde (MDA) were determined in Asian swimming crab Charybdis japonica exposed to different cadmium (Cd) levels including 0 mg/L in control group ,0 .0025 mg/L and 0 .005 mg/L in LC groups ,0 .05 mg/L and 0 .1 mg/L in MC groups ,and 0 .25 mg/L and 0 .5 mg/L in HC groups 0 ,1 ,3 ,5 ,7 ,10 ,15 and 30 days after the exposure .The Cd accumulation was detected in the hepatopancreas ,muscle ,ovary ,and gill at the 30th day .The results showed that the SOD and CAT activities were found to be elevated first and then to be declined after Cd exposure ,with more rapid induction in MC groups ,and HC groups than in LC groups .On days 15 and 30 ,the activities of SOD and CAT were significantly lower in the treatment groups than those in the control group except in 0 .0025 mg/L group ,with significant negative correlation with Cd concentration(P<0 .05) .The MDA contents were first increased ,then declined and elevated in the groups with Cd concentrations of 0 .005 ,0 .05 and 0 .1 mg/L .However ,the constant increase in MDA contents were observed in HC groups ,even though there was no significant change in MDA contents in the 0 .0025 mg/L group .Until the 10th day after exposure ,the MDA contents were significantly higher in the treat-ment groups than that in the control ,showing a positive correlation with Cd concentration ,except in 0 .0025 mg/L group .There was more Cd accumulation in the hepatopancreas and gill ,while minute a-mounts of Cd was detected in the ovary and muscle in the HC groups .Significant effect-dosage relation-ships were observed between the accumulation content and Cd concentration in the hepatopancreas in the treatment groups .There were more Cd accumulation in the gills than that in hepatopancreas in MC and HC groups .