CAJ | 학술논문

自杀基因靶向疗法在恶性肿瘤的治疗方法中占有重要的位置。众多体内体外试验已经证明了自杀基因的肿瘤杀伤效应。目前，相关的临床试验也证实HSV-tk/GCV，CD/5-FC等自杀基因系统具有良好的抗肿瘤效应。然而在临床推广应用上还有很多问题需要解决，如基因靶向转移困难、基因转染效率较低、基因转移后的表达缺乏有效的调控手段和基因杀伤效应较低且难以控制等，这些问题均有待于我们在今后的研究中进一步解决。本文将从自杀基因的种类、载体、作用机制、旁观者效应和临床发展前景，尤其是纳米粒载体和肿瘤干细胞理论的应用等方面进行综述。
자살기인파향요법재악성종류적치료방법중점유중요적위치。음다체내체외시험이경증명료자살기인적종류살상효응。목전，상관적림상시험야증실HSV-tk/GCV，CD/5-FC등자살기인계통구유량호적항종류효응。연이재림상추엄응용상환유흔다문제수요해결，여기인파향전이곤난、기인전염효솔교저、기인전이후적표체결핍유효적조공수단화기인살상효응교저차난이공제등，저사문제균유대우아문재금후적연구중진일보해결。본문장종자살기인적충류、재체、작용궤제、방관자효응화림상발전전경，우기시납미립재체화종류간세포이론적응용등방면진행종술。
Suicide gene therapy is an important method of gene therapy for cancer.It has been successfully used in a large number of in vitro and in vivo studies.The two major suicide gene therapeutic strategies currently pursued are:herpes simplex virus thymidine kinase/ganciclovir(HSV-tk/GCV)and cytosine deaminase/5 -fluorocytosine(CD/5-FC).In this review,we summarize the different suicide gene systems and gene delivery vectors addressed to canc-er,and the different strategies that have been used with suicide gene therapy and provide some insights into the future directions of this approach,particularly towards nanoparticle carrier and cancer stem cells.