CAJ | 학술논문

目的：探讨自噬对人胆管癌HCCC-9810细胞125 I粒子内放疗敏感性的影响。方法：用MTT检测不同照射剂量下125 I粒子对HCCC-9810细胞增殖活性的影响。通过电镜检测自噬体变化，用Western blot检测LC3来观察不同照射剂量下125 I粒子对HCCC-9810细胞自噬发生的影响。3-MA抑制自噬后检测125 I粒子对HCCC-9810细胞增殖活性的影响。结果：125 I粒子照射HCCC-9810细胞后细胞活性明显下降并呈剂量相关性。电镜检测和LC3-Ⅱ蛋白印迹显示内放疗照射后HCCC-9810细胞自噬明显增加。3-MA抑制自噬后125 I粒子内放疗对HCCC-9810细胞的抑制作用显著增强。结论：125 I粒子内放疗后HCCC-9810细胞自噬明显增加，抑制自噬后125 I粒子内放疗对HCCC-9810细胞生长的抑制作用显著增强。
목적：탐토자서대인담관암HCCC-9810세포125 I입자내방료민감성적영향。방법：용MTT검측불동조사제량하125 I입자대HCCC-9810세포증식활성적영향。통과전경검측자서체변화，용Western blot검측LC3래관찰불동조사제량하125 I입자대HCCC-9810세포자서발생적영향。3-MA억제자서후검측125 I입자대HCCC-9810세포증식활성적영향。결과：125 I입자조사HCCC-9810세포후세포활성명현하강병정제량상관성。전경검측화LC3-Ⅱ단백인적현시내방료조사후HCCC-9810세포자서명현증가。3-MA억제자서후125 I입자내방료대HCCC-9810세포적억제작용현저증강。결론：125 I입자내방료후HCCC-9810세포자서명현증가，억제자서후125 I입자내방료대HCCC-9810세포생장적억제작용현저증강。
Objective:To investigate the role of autophagy in 125 I seeds brachytherapy-induced cytotoxicity in hu-man HCCC-9810 cells.Methods:The growth inhibition of HCCC-9810 cells induced by brachytherapy with 125 I seeds was assessed with methyl thialy terazolium (MTT)assay.The effects of 125 I seeds on autophagy were observed with electron microscopy (EM)and microtubule -associated protein 1 light chain 3 (LC3 )detected by Western blot.The autophagic inhibitor 3 -methylademine (3 -MA)was used to characterize the effect of autophagy on the brachytherapy-induced cytotoxicity.Results:Brachytherapy with low dose rate of 125 I seeds decreased the viability of HCCC-9810 cells in a dose-dependent manner.Autophagy induced by brachytherapy was evidenced by EM.West-ern blot analysis indicated that brachytherapy with low dose rate of 125 I seeds also augmented the inhibitory effect of brachytherapy with 125 I seeds on the sensitization of HCCC-9810 cells.Conclusion:Brachytherapy with 125 I seeds in-duces autophagy in human HCCC-9810 cells and autophagic inhibitors can increase the cytotoxicity of brachytherapy to cholangiocarcinoma cells.