CAJ | 학술논문

目的：探讨非酒精性脂肪性肝病（NAFLD）血液标本的采集和保存及生物样本库的建立和信息化管理方法。方法收集2009年10月-2013年10月经B超及临床血液学检测诊断为NAFLD的1226例患者的全血标本，进行生化指标测定、血浆及全血基因组DNA提取，并检测DNA的浓度和纯度，分别放至-80℃超低温冰箱储存备用，同时建立一套信息管理系统用于NAFLD生物样本库的管理。结果1226例NAFLD患者的全血标本，其中包括双生子标本83例及家系标本100例。血浆及DNA的提取成功率均为100％，并随机抽取100份 DNA 进行检测，结果表明所收集标本能够满足后续试验要求。结论该研究成功建立了NAFLD生物样本库，拥有标准化的信息管理系统，对标本进行质量控制和信息化管理，为进一步研究NAFLD奠定了良好的基础。
목적：탐토비주정성지방성간병（NAFLD）혈액표본적채집화보존급생물양본고적건립화신식화관리방법。방법수집2009년10월-2013년10월경B초급림상혈액학검측진단위NAFLD적1226례환자적전혈표본，진행생화지표측정、혈장급전혈기인조DNA제취，병검측DNA적농도화순도，분별방지-80℃초저온빙상저존비용，동시건립일투신식관리계통용우NAFLD생물양본고적관리。결과1226례NAFLD환자적전혈표본，기중포괄쌍생자표본83례급가계표본100례。혈장급DNA적제취성공솔균위100％，병수궤추취100빈 DNA 진행검측，결과표명소수집표본능구만족후속시험요구。결론해연구성공건립료NAFLD생물양본고，옹유표준화적신식관리계통，대표본진행질량공제화신식화관리，위진일보연구NAFLD전정료량호적기출。
Objective To investigate the collection and preservation of blood specimens from patients with nonalcoholic fatty liver disease (NAFLD)and the establishment and information management of biobank.Methods Whole blood samples were collected from 1226 pa-tients who were diagnosed with NAFLD based on B-mode ultrasound and blood tests from October 2009 to October 2013.Biochemical pa-rameters were measured.Plasma and whole-blood genomic DNA was extracted from the samples,and the purity and concentration of DNA were determined.Specimens were preserved in a refrigerator (-80℃).An information management system for NAFLD biobank was estab-lished.Results Specimens of 1226 NAFLD patients,including those of 83 twins and 100 families,were collected.The success rate was 100% for extraction of plasma and whole-blood genomic DNA.One hundred DNA samples were randomly selected for testing,and the re-sults showed that the collected specimens met the requirements of following experiments.Conclusion The NAFLD Biobank has been suc-cessfully established in this study.It has the standard information management system and enables the quality control and information man-agement of specimens,laying a solid foundation for further research on NAFLD.