临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2014年
9期
873-876
,共4页
刘力伟%李嘉嘉%朱立新%刘波
劉力偉%李嘉嘉%硃立新%劉波
류력위%리가가%주립신%류파
肝炎,乙型,慢性%胎血%杀伤细胞%细胞增殖%免疫活性
肝炎,乙型,慢性%胎血%殺傷細胞%細胞增殖%免疫活性
간염,을형,만성%태혈%살상세포%세포증식%면역활성
hepatitis B,chronic%fetal blood%killer cells%cell proliferation%immunocompetence
目的：观察脐血和不同年龄段慢性乙型肝炎（CHB）患者细胞因子诱导的杀伤细胞（CIK细胞）的体外增殖能力及杀伤活性的特点。方法采集2012年1月至2012年12月在解放军第一0五医院分娩的健康胎儿的脐带血5份（A组）；采集20例CHB患者的外周血，分为2组，即B组12例（年龄20～35岁），C组8例（年龄＞35岁）。采用Ficoll两步分离法分离出单个核细胞，在体外应用细胞因子诱导培养成CIK细胞，检测CIK细胞的免疫表型，噻唑蓝比色法测定其对白血病K562细胞的杀伤活性，采用t检验分析不同组CIK细胞增殖倍数，卡方检验分析杀伤活性率的差异。结果第15天时，B组体外CIK细胞增殖达到（589．15±237．60）倍，A组为（600．93±249．10）倍，2组相比差异无统计学意义（P＞0．05）；而C组为（370．45±165．2）倍，与A、B组相比差异有统计学意义（P＜0.05）。A、B、C 3组CIK细胞对K562的杀伤率分别为（77．3±5．1）％、（54．5±3．5）％、（44．1±3．4）％，A组与B、C 2组相比差异具有统计学意义（P＜0.05、P＜0．01）。结论脐血来源的CIK细胞体外增殖快，杀伤活性强；大于35岁的CHB患者CIK细胞体外增殖能力明显降低，不宜接受自体CIK细胞移植。
目的：觀察臍血和不同年齡段慢性乙型肝炎（CHB）患者細胞因子誘導的殺傷細胞（CIK細胞）的體外增殖能力及殺傷活性的特點。方法採集2012年1月至2012年12月在解放軍第一0五醫院分娩的健康胎兒的臍帶血5份（A組）；採集20例CHB患者的外週血，分為2組，即B組12例（年齡20～35歲），C組8例（年齡＞35歲）。採用Ficoll兩步分離法分離齣單箇覈細胞，在體外應用細胞因子誘導培養成CIK細胞，檢測CIK細胞的免疫錶型，噻唑藍比色法測定其對白血病K562細胞的殺傷活性，採用t檢驗分析不同組CIK細胞增殖倍數，卡方檢驗分析殺傷活性率的差異。結果第15天時，B組體外CIK細胞增殖達到（589．15±237．60）倍，A組為（600．93±249．10）倍，2組相比差異無統計學意義（P＞0．05）；而C組為（370．45±165．2）倍，與A、B組相比差異有統計學意義（P＜0.05）。A、B、C 3組CIK細胞對K562的殺傷率分彆為（77．3±5．1）％、（54．5±3．5）％、（44．1±3．4）％，A組與B、C 2組相比差異具有統計學意義（P＜0.05、P＜0．01）。結論臍血來源的CIK細胞體外增殖快，殺傷活性彊；大于35歲的CHB患者CIK細胞體外增殖能力明顯降低，不宜接受自體CIK細胞移植。
목적：관찰제혈화불동년령단만성을형간염（CHB）환자세포인자유도적살상세포（CIK세포）적체외증식능력급살상활성적특점。방법채집2012년1월지2012년12월재해방군제일0오의원분면적건강태인적제대혈5빈（A조）；채집20례CHB환자적외주혈，분위2조，즉B조12례（년령20～35세），C조8례（년령＞35세）。채용Ficoll량보분리법분리출단개핵세포，재체외응용세포인자유도배양성CIK세포，검측CIK세포적면역표형，새서람비색법측정기대백혈병K562세포적살상활성，채용t검험분석불동조CIK세포증식배수，잡방검험분석살상활성솔적차이。결과제15천시，B조체외CIK세포증식체도（589．15±237．60）배，A조위（600．93±249．10）배，2조상비차이무통계학의의（P＞0．05）；이C조위（370．45±165．2）배，여A、B조상비차이유통계학의의（P＜0.05）。A、B、C 3조CIK세포대K562적살상솔분별위（77．3±5．1）％、（54．5±3．5）％、（44．1±3．4）％，A조여B、C 2조상비차이구유통계학의의（P＜0.05、P＜0．01）。결론제혈래원적CIK세포체외증식쾌，살상활성강；대우35세적CHB환자CIK세포체외증식능력명현강저，불의접수자체CIK세포이식。
Objective To observe the in vitro proliferation capacity and killing efficacy of cytokine-induced killer (CIK)cells from um-bilical cord blood (UCB)and versus chronic hepatitis B (CHB)patients of different ages.Methods Five samples of UCB from healthy fe-tuses born in our hospital from January to December,2012,as well as 20 samples of peripheral blood from CHB patients,were divided into group A (5 samples of UCB),group B (12 samples of peripheral blood from CHB patients aged 20-35 years),and group C (8 samples of peripheral blood from CHB patients aged over 35 years).Mononuclear cells were separated by Ficoll-Hypaque centrifugation.Peripheral blood mononuclear cells were induced into CIK cells in the presence of cytokines.The immunophenotypes of CIK cells were determined by flow cytometry.The killing efficacy of CIK cells against K562 cells was measured by MTT assay.Results On day 15,CIK cells from group B multiplied 589.15 ±237.6 times,versus 600.93 ±249.1 times for group A (P>0.05);CIK cells from group C multiplied 370.45 ± 165.2 times,which were significantly lower than those for groups A and B (P<0.05).The killing efficacy of CIK cells from groups A,B, and C against K562 cells was (77.3 ±5.1)%,(54.5 ±3.5)%,and (44.1 ±3.4)%,respectively;there were significant differences in killing efficacy between group A and groups B and C (P<0.01).Conclusion UCB-derived CIK cells have high in vitro proliferation ca-pacity and killing efficacy.The in vitro proliferation capacity of CIK cells declines in CHB patients aged over 35 years,so autologous trans-plantation of CIK cells is not suitable for this group of patients.
