CAJ | 학술논문

目的：探讨缺氧条件下Galectin-1对口腔鳞癌细胞Tca8113的生物学行为的影响。方法：siRNA干扰Galectin-1在Tca8113细胞中的表达；采用MTT法检测细胞的增殖；Real time-PCR分析Galectin-1 mR-NA和Bcl-2 mRNA的表达；Transwell小室观察各组细胞的侵袭能力；免疫荧光分析缺氧条件下细胞的凋亡情况。结果：siRNA-Galectin-l干扰组和200μmol/L氯化钴处理组的细胞出现了增殖抑制；用200μmol/L氯化钴处理Tca8113细胞后，Galectin-l mRNA的表达量上调（0．91±0．11，P＜0．05）；siRNA＋Tca8113细胞组Bcl-2 mRNA的表达量明显减低（0．16±0．02，P＜0．05）；siRNA-Galectin-l干扰组迁移出的细胞数（31±6）明显低于200μmol/L氯化钴＋Tca8113细胞组（43±6）和Tca8113细胞组（51±8，P＜0．05）；免疫荧光结果所示，siRNA＋200μmol/L氯化钴＋Tca8113细胞组的荧光强度最弱。结论：Galectin-1是反应肿瘤细胞缺氧的一个较为敏感的指标，可利用Galectin-1作为内在的缺氧标志物。
목적：탐토결양조건하Galectin-1대구강린암세포Tca8113적생물학행위적영향。방법：siRNA간우Galectin-1재Tca8113세포중적표체；채용MTT법검측세포적증식；Real time-PCR분석Galectin-1 mR-NA화Bcl-2 mRNA적표체；Transwell소실관찰각조세포적침습능력；면역형광분석결양조건하세포적조망정황。결과：siRNA-Galectin-l간우조화200μmol/L록화고처리조적세포출현료증식억제；용200μmol/L록화고처리Tca8113세포후，Galectin-l mRNA적표체량상조（0．91±0．11，P＜0．05）；siRNA＋Tca8113세포조Bcl-2 mRNA적표체량명현감저（0．16±0．02，P＜0．05）；siRNA-Galectin-l간우조천이출적세포수（31±6）명현저우200μmol/L록화고＋Tca8113세포조（43±6）화Tca8113세포조（51±8，P＜0．05）；면역형광결과소시，siRNA＋200μmol/L록화고＋Tca8113세포조적형광강도최약。결론：Galectin-1시반응종류세포결양적일개교위민감적지표，가이용Galectin-1작위내재적결양표지물。
Objective:The biological effects of Galectin-l for oral squamous cell carcinoma cells in hypoxic envi-ronment were investigated.Methods:To interfere the expression of Galectin-1 in cells with siRNA,the proliferation of cells was detected by MTT methods;the expression of Galectin-1 mRNA and Bcl-2 mRNA were analysed by real time-PCR,using Transwell to detect the invasion ability;to analyse the condition of cell apoptosis in hypoxic by im-munofluorescence.Results:siRNA-Galectin-l group and 200μmol/L CoCl2 group appeared the inhibition of prolif-eration,the expression of Galectin-1 mRNA was significantly increased in 200μmol/L CoCl2 group (0.91 ±0.11,P<0.05),the expression of Bcl-2 mRNA was significantly reduced in siRNA group (0.16 ±0.02,P<0.05);the cell counts of siRNA-Galectin-l interference group (31 ±6)was significantly lower than 200μmol/L CoCl2 group (43 ±6)was Tca8113 cell group (51 ±8,P<0.05),the fluorescence intensity of siRNA and 200μmol/L CoCl2 and Tca8113 cell group were the weakest.Conclusion:Galectin-1 was a sensitive indicator with tumor cells in hypoxic environment,Galectin-1 maybe used as an inner oxygen marker.