CAJ | 학술논문

目的：观察佐剂性关节炎大鼠的骨密度、血清骨保护素( OPG)及核因子-κB受体激活因子配体( RANKL)的表达。方法：将30只SD大鼠随机均分为正常对照组、模型1组、模型2组,除正常对照组外,其余大鼠均制成佐剂性关节炎模型。造模后第21天处死模型1组,第28天处死正常对照组和模型2组。以双能X线吸收测定法测量整体右侧胫骨和距胫骨远端约1 cm兴趣区检测骨密度；ELISA方法检测血清OPG、RANKL的表达。结果：模型1组大鼠兴趣区的骨密度明显低于正常对照组(P<0.01)；模型2组大鼠兴趣区的骨密度均较模型1组和正常对照组显著降低(P<0.01)；3组大鼠整体右侧胫骨骨密度值差异无统计学意义(P>0.05)。模型1组、模型2组血清OPG、RANKL表达及OPG/RANKL比值与正常对照组差异均有统计学意义(P<0.01)。同时模型1组与模型2组血清 OPG、RANKL 表达及 OPG/RANKL 比值差异亦均有统计学意义(P <0.01)。结论：佐剂性关节炎大鼠胫骨远端骨丢失的发生在整体胫骨之前。造模后第21天和第28天的 OPG 表达降低, RANKL表达升高,OPG/RANKL比值降低。
목적：관찰좌제성관절염대서적골밀도、혈청골보호소( OPG)급핵인자-κB수체격활인자배체( RANKL)적표체。방법：장30지SD대서수궤균분위정상대조조、모형1조、모형2조,제정상대조조외,기여대서균제성좌제성관절염모형。조모후제21천처사모형1조,제28천처사정상대조조화모형2조。이쌍능X선흡수측정법측량정체우측경골화거경골원단약1 cm흥취구검측골밀도；ELISA방법검측혈청OPG、RANKL적표체。결과：모형1조대서흥취구적골밀도명현저우정상대조조(P<0.01)；모형2조대서흥취구적골밀도균교모형1조화정상대조조현저강저(P<0.01)；3조대서정체우측경골골밀도치차이무통계학의의(P>0.05)。모형1조、모형2조혈청OPG、RANKL표체급OPG/RANKL비치여정상대조조차이균유통계학의의(P<0.01)。동시모형1조여모형2조혈청 OPG、RANKL 표체급 OPG/RANKL 비치차이역균유통계학의의(P <0.01)。결론：좌제성관절염대서경골원단골주실적발생재정체경골지전。조모후제21천화제28천적 OPG 표체강저, RANKL표체승고,OPG/RANKL비치강저。
Objective:To observe the bone mineral density and the expression of blood serum osteoprotegerin ( OPG )/receptor activator of nuclear factor-κB ligand( RANKL) protein in adjuvant arthritis rats. Methods:Thirty SD rats were randomly divided into normal control group,model 1 group and model 2 group. Except the normal control group, other rats were made of adjuvant arthritis model. At the 21st day,the bone mineral density of the whole right side of the tibia and about 1 cm from the distal tibia called interest region in model 1 group were detected with dualenergy X-ray absorptoimetry. At the 28th day,the bone mineral density of the whole right side of the tibia and about 1 cm from the distal tibia called interest region in model 2 group and normal group were detected. At the same time,the expression of blood serum OPG/RANKL of adjuvant arthritis rats was inspected by enzyme-linked immuno sorbent assay. Results:The bone mineral density of interest region of model 1 group were lower than that of normal group(P<0. 01). The interest region of model 2 group significantly reduced compared with the normal and model 1 groups(P<0. 01). The overall right tibia bone mineral density had no significant difference(P>0. 05). The expression of blood serum OPG,RANKL and the OPG/RANKL ratio in model 1 and model 2 group were significantly different with the normal group(P<0. 01). At the same time,the expression of blood serum OPG,RANKL and the OPG/RANKL ratio in model 2 were significantly different with model 1 group(P<0. 01). Conclusions:Adjuvant arthritis rats lost distal tibial bone earlier than the whole tibial. The blood serum OPG expression is decreased,the blood serum RANKL is increased,and the ratio of OPG/RANKL is decreased after modeling 21 days and 28 days.