CAJ | 학술논문

目的：探讨锌指蛋白185（zinc finger protein 185，ZNF185）在人胶质瘤细胞迁移中的作用。方法：Western blot检测ZNF185在神经胶质瘤及瘤旁正常组织的蛋白表达，克隆ZNF185基因转染人胶质瘤细胞，免疫荧光染色检测ZNF185的细胞定位，细胞划线和Transwell小室法检测ZNF185蛋白对细胞迁移的影响。结果：与瘤旁正常脑组织相比，ZNF185在人胶质瘤组织和细胞系中的表达显著降低；ZNF185蛋白主要分布于人胶质瘤细胞的细胞质、细胞膜，并与肌动蛋白纤维共定位；过表达ZNF185显著抑制人胶质瘤细胞的迁移。结论：ZNF185通过结合肌动蛋白纤维发挥抑制人胶质瘤细胞迁移的作用。
목적：탐토자지단백185（zinc finger protein 185，ZNF185）재인효질류세포천이중적작용。방법：Western blot검측ZNF185재신경효질류급류방정상조직적단백표체，극륭ZNF185기인전염인효질류세포，면역형광염색검측ZNF185적세포정위，세포화선화Transwell소실법검측ZNF185단백대세포천이적영향。결과：여류방정상뇌조직상비，ZNF185재인효질류조직화세포계중적표체현저강저；ZNF185단백주요분포우인효질류세포적세포질、세포막，병여기동단백섬유공정위；과표체ZNF185현저억제인효질류세포적천이。결론：ZNF185통과결합기동단백섬유발휘억제인효질류세포천이적작용。
Objective:To explore the effect of Zinc finger protein 185 (ZNF185) on the migration of human gliocytoma cells. Methods: ZNF185 protein expression in human gliocytoma and its adjacent normal tissues was detected through Western blot. The ZNF185 gene was cloned and transfected into the human gliocytoma cell line SF767. ZNF185 cells were located through immunofluo-rescence staining, and cell migration was analyzed using the cell scoring method. Results:CZNF185 protein expression was lower in the human gliocytoma cell line than in normal para-neoplastic tissues. ZNF185 protein was mainly expressed in the cytoplasm and cell membrane of the human gliocytoma cells and was co-located with F-actin. ZNF185 overexpression repressed the migration of human gliocytoma cells. Conclusion:ZNF185 represses the migration of human gliocytoma cells by binding to F-actin.