中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2014年
17期
1080-1083
,共4页
前列腺癌%miR-149%FOXM1%生长%侵袭
前列腺癌%miR-149%FOXM1%生長%侵襲
전렬선암%miR-149%FOXM1%생장%침습
prostate carcinoma%miR-149%FOXM1%cell growth%invasion
目的：探讨miR-149对前列腺癌细胞生长和侵袭的影响，并研究miR-149的靶基因及其功能。方法：利用实时荧光定量PCR检测前列腺癌和癌旁组织miR-149和FOXM1 mRNA表达水平。在人前列腺癌细胞系PC3和DU145细胞中瞬时转染miR-149模拟物和阴性对照miRNA，运用平板克隆形成和Transwell侵袭实验检测细胞生长和侵袭。在细胞中分别转染miR-149靶基因FOXM1的siRNA和siRNA阴性对照，利用Western blot检测FOXM1蛋白表达情况，并检测细胞的克隆形成和侵袭能力。结果：在前列腺癌中miR-149低表达（P<0.01），FOXM1 mRNA高表达（P<0.01）。与对照组细胞相比，转染miR-149模拟物的PC3和DU145细胞克隆数目减少（P<0.01），发生侵袭的细胞减少（P<0.01）；FOXM1蛋白水平在转染miR-149模拟物的PC3（P<0.01）和DU145细胞（P<0.05）中较对照组细胞降低。转染FOXM1 siRNA的PC3和DU145细胞中FOXM1蛋白水平较对照组降低，且克隆形成和侵袭能力较对照组明显降低（P<0.01）。结论：miR-149通过靶向FOXM1抑制前列腺癌细胞生长和侵袭，发挥着抑癌基因的作用，可作为前列腺癌分子治疗的有效靶点。
目的：探討miR-149對前列腺癌細胞生長和侵襲的影響，併研究miR-149的靶基因及其功能。方法：利用實時熒光定量PCR檢測前列腺癌和癌徬組織miR-149和FOXM1 mRNA錶達水平。在人前列腺癌細胞繫PC3和DU145細胞中瞬時轉染miR-149模擬物和陰性對照miRNA，運用平闆剋隆形成和Transwell侵襲實驗檢測細胞生長和侵襲。在細胞中分彆轉染miR-149靶基因FOXM1的siRNA和siRNA陰性對照，利用Western blot檢測FOXM1蛋白錶達情況，併檢測細胞的剋隆形成和侵襲能力。結果：在前列腺癌中miR-149低錶達（P<0.01），FOXM1 mRNA高錶達（P<0.01）。與對照組細胞相比，轉染miR-149模擬物的PC3和DU145細胞剋隆數目減少（P<0.01），髮生侵襲的細胞減少（P<0.01）；FOXM1蛋白水平在轉染miR-149模擬物的PC3（P<0.01）和DU145細胞（P<0.05）中較對照組細胞降低。轉染FOXM1 siRNA的PC3和DU145細胞中FOXM1蛋白水平較對照組降低，且剋隆形成和侵襲能力較對照組明顯降低（P<0.01）。結論：miR-149通過靶嚮FOXM1抑製前列腺癌細胞生長和侵襲，髮揮著抑癌基因的作用，可作為前列腺癌分子治療的有效靶點。
목적：탐토miR-149대전렬선암세포생장화침습적영향，병연구miR-149적파기인급기공능。방법：이용실시형광정량PCR검측전렬선암화암방조직miR-149화FOXM1 mRNA표체수평。재인전렬선암세포계PC3화DU145세포중순시전염miR-149모의물화음성대조miRNA，운용평판극륭형성화Transwell침습실험검측세포생장화침습。재세포중분별전염miR-149파기인FOXM1적siRNA화siRNA음성대조，이용Western blot검측FOXM1단백표체정황，병검측세포적극륭형성화침습능력。결과：재전렬선암중miR-149저표체（P<0.01），FOXM1 mRNA고표체（P<0.01）。여대조조세포상비，전염miR-149모의물적PC3화DU145세포극륭수목감소（P<0.01），발생침습적세포감소（P<0.01）；FOXM1단백수평재전염miR-149모의물적PC3（P<0.01）화DU145세포（P<0.05）중교대조조세포강저。전염FOXM1 siRNA적PC3화DU145세포중FOXM1단백수평교대조조강저，차극륭형성화침습능력교대조조명현강저（P<0.01）。결론：miR-149통과파향FOXM1억제전렬선암세포생장화침습，발휘착억암기인적작용，가작위전렬선암분자치료적유효파점。
Objective:To investigate the effects of the miR-149 on the growth and invasion of prostate carcinoma cells. Meth-ods:Real-time fluorescence quantitative polymerase chain reaction was performed to detect the expression of miR-149 on prostate car-cinoma tissues and paraneoplastic tissues. The PC3 and DU145 cells were transfected with miR-149 mimics and negative controls. The cell growth and invasion abilities were tested in terms of colony formation and via Transwell invasion assay. The cells were transfected with the siRNA of the target gene FOXM1 and siRNA control. Western blot was used to detect the expression of FOXM1. The cell colo-ny formation and invasion ability were also detected. Results:Compared with the paraneoplastic tissues, miR-149 was down-regulated in the prostate carcinoma tissues (P<0.01), and the FOXM1 mRNA was highly expressed (P<0.01). PC3 and DU145 cells with miR-149 mimics had only a few colonies and invading cells (P<0.01). Moreover, PC3 (P<0.01) and DU145 (P<0.05) with miR-149 mimics had a low protein level of FOXM1. The FOXM1 expression was knocked down by the siRNA of FOXM1 in the PC3 and the DU145 cells (P<0.01). The knocking down of FOXM1 resulted in an inhibition of the cell colony formation and invasion abilities (P<0.01). Conclusion:The miR-149 inhibits prostate carcinoma cell growth and invasion by suppressing the FOXM1. Our data suggest that miR-149 may function as an effective tool for the molecular treatment of prostate cancer.
