CAJ | 학술논문

目的：研究结核分枝杆菌脂多糖抗原（LAM）和结核分枝杆菌重组38KD 蛋白抗原（r38KD）在结核病血清学检测中的效果，探索更有价值的结核血清学诊断方法。方法应用酶联免疫吸附试验对100例结核病患者血清、100例健康人血清中的抗结核抗体进行检测，并对检测结果进行统计分析。结果结核组中抗 LAM 抗体和抗38KD 抗体均目的研究结核分枝杆菌脂多糖抗原（LAM）和结核分枝杆菌重组38KD 蛋白抗原（r38KD）在结核病血清学检测中的效果，探索更有价值的结核血清学诊断方法。方法应用酶联免疫吸附试验对100例结核病患者血清、100例健康人血清中的抗结核抗体进行检测，并对检测结果进行统计分析。结果结核组中抗 LAM 抗体和抗38KD 抗体均显著高于非结核对照组（ P ﹤0.05），且两种抗原对结核病检测具有互补性。结论将 LAM 和38KD 两种不同的结核分枝抗原进行组合可提高检测的特异度，有利于结核病的血清学诊断。
목적：연구결핵분지간균지다당항원（LAM）화결핵분지간균중조38KD 단백항원（r38KD）재결핵병혈청학검측중적효과，탐색경유개치적결핵혈청학진단방법。방법응용매련면역흡부시험대100례결핵병환자혈청、100례건강인혈청중적항결핵항체진행검측，병대검측결과진행통계분석。결과결핵조중항 LAM 항체화항38KD 항체균목적연구결핵분지간균지다당항원（LAM）화결핵분지간균중조38KD 단백항원（r38KD）재결핵병혈청학검측중적효과，탐색경유개치적결핵혈청학진단방법。방법응용매련면역흡부시험대100례결핵병환자혈청、100례건강인혈청중적항결핵항체진행검측，병대검측결과진행통계분석。결과결핵조중항 LAM 항체화항38KD 항체균현저고우비결핵대조조（ P ﹤0.05），차량충항원대결핵병검측구유호보성。결론장 LAM 화38KD 량충불동적결핵분지항원진행조합가제고검측적특이도，유리우결핵병적혈청학진단。
Objective To evaluate the significance of LAM and r38KD in serodiagnosis of tuberculosis(TB),and to develop more effec-tive diagnostic methods for tuberculosis. Methods A total of 100 serum samples from patients with tuberculosis and 100 serum samples of healthy controls were detected for antibodies against these two antigens by ELISA,and the data were evaluated by SPSS software. Results The levels of anti - LAM and anti - 38KD antibodies in patients of TB group were significantly higher than those of control group( P ﹤ 0. 05),and these two antigens had some complementary effect with each other in diagnosis of tuberculosis. Conclusion The combined application of LAM and 38KD antigens may increase the specificity in diagnosis of tuberculosis,and it may be beneficial in serodiagnosis of TB.