CAJ | 학술논문

目的：探讨原代培养人脑多形性胶质母细胞瘤细胞的方法，观察培养细胞的生长活性，建立人脑多形性胶质母细胞瘤细胞体外实验模型。方法采用酶消化法原代培养14例人脑多形性胶质母细胞瘤细胞，用倒置相差显微镜和荧光显微镜观察增殖过程中细胞的形态学特点，台盼蓝染色法观察生长活性，描记生长曲线以研究细胞的生长特性。结果12例原代培养成功，第4代以后肿瘤细胞纯度达98%以上。培养成功的细胞状态稳定，增殖活跃，有明显的对数生长期。结论采用酶消化法可成功原代培养人脑多形性胶质母细胞瘤细胞，酶消化法加划除法可获得高纯度的肿瘤细胞，成功率较高，适宜体外培养细胞。
목적：탐토원대배양인뇌다형성효질모세포류세포적방법，관찰배양세포적생장활성，건립인뇌다형성효질모세포류세포체외실험모형。방법채용매소화법원대배양14례인뇌다형성효질모세포류세포，용도치상차현미경화형광현미경관찰증식과정중세포적형태학특점，태반람염색법관찰생장활성，묘기생장곡선이연구세포적생장특성。결과12례원대배양성공，제4대이후종류세포순도체98%이상。배양성공적세포상태은정，증식활약，유명현적대수생장기。결론채용매소화법가성공원대배양인뇌다형성효질모세포류세포，매소화법가화제법가획득고순도적종류세포，성공솔교고，괄의체외배양세포。
Objective To investigate the method for primary human glioblastoma multiforme(GBM)cells culture,and establish an ex-perimental model for studying glioblastoma in vitro. Methods GBM cells isolated from 14 glioblastoma patients were cultured primarily by the en-zymatic digestion culture method. The cell morphology was observed with inverted phase contrast microscope and fluorescence microscope,and the growth curve was analyzed by trypan blue staining. Results Twelve prmiary cultures of GBM cells were successfully established. The percentage of tumor cells was over 98% after 4 tranfers. The cultured cells had the proliferative ability and logarithmic growth phase. Conclusion The hu-man GBM cells can be cultured and highly purified GBM cells can be obtained by enzymatic digestion culture method.