CAJ | 학술논문

目的：研究夜交藤提取物对大鼠完全性脑缺血再灌注损伤的保护作用，并初步探讨其保护机制。方法取 wistar 大鼠，采用四血管阻断法制备完全性脑缺血再灌注损伤模型，将成模大鼠随机分为夜交藤组（灌胃给予夜交藤提取物）和模型组（灌胃生理盐水），另设假手术组（灌胃生理盐水），每组10只。再灌注72 h 后，断头取血，比色法测血清一氧化氮合成酶（NOS）、超氧化物岐化酶（SOD）的活性及一氧化氮（NO）、丙二醛（MDA）的含量；取脑缺血组织，测定脑组织兴奋性氨基酸（EAA）含量。结果与模型组相比，夜交藤组大鼠血清 SOD 的活性升高，NOS 的活性及 NO、MDA含量均降低，能显著减轻缺血、缺氧造成的 EAA 含量增加，抑制 EAA 所导致的兴奋性神经毒性。结论夜交藤提取物对脑缺血再灌注损伤具有一定的保护作用，其保护机制与夜交藤提取物抑制 NO 释放及增强氧自由基的清除有关。
목적：연구야교등제취물대대서완전성뇌결혈재관주손상적보호작용，병초보탐토기보호궤제。방법취 wistar 대서，채용사혈관조단법제비완전성뇌결혈재관주손상모형，장성모대서수궤분위야교등조（관위급여야교등제취물）화모형조（관위생리염수），령설가수술조（관위생리염수），매조10지。재관주72 h 후，단두취혈，비색법측혈청일양화담합성매（NOS）、초양화물기화매（SOD）적활성급일양화담（NO）、병이철（MDA）적함량；취뇌결혈조직，측정뇌조직흥강성안기산（EAA）함량。결과여모형조상비，야교등조대서혈청 SOD 적활성승고，NOS 적활성급 NO、MDA함량균강저，능현저감경결혈、결양조성적 EAA 함량증가，억제 EAA 소도치적흥강성신경독성。결론야교등제취물대뇌결혈재관주손상구유일정적보호작용，기보호궤제여야교등제취물억제 NO 석방급증강양자유기적청제유관。
Objective To explore the therapeutic effects of Caulis Polygoni Multiflori extract on cerebral ischemia/ reperfusion injury and its mechanism. Methods The global cerebral ischemia/ reperfusion injury model was established by the four - Vesse1 occ1usion(4VO)method, and then the model animals were divided into Caulis Polygoni Multiflori group,model group and sham operation group,each group of 10. The activi-ties of NOS and SOD,and the levels of NO and MDA were measured after 72 hours of the experiment. Moreover,the excitatory amino acid(EAA) in ischemic brain tissue were Examined. Results Caulis Polygoni Multiflori extract increased the activities of SOD,lowered the levels of NOS, NO and MDA,reduced the inerease of EAA content caused by ischemia and hypoxia,and inhibited the excitatory neurotoxieity caused by EAA. Conclusion Caulis Polygoni Multiflori extract on cerebral ischemia reperfusion injury has a protective effect,the protection mechanism and Caulis Polygoni Multiflori extract inhibited the NO release and scavenging oxygen free radical.