临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2014年
18期
1561-1563
,共3页
邵坤%贺诗文%刘倩%王刘峰%郝晓霏%吕汇
邵坤%賀詩文%劉倩%王劉峰%郝曉霏%呂彙
소곤%하시문%류천%왕류봉%학효비%려회
梅毒%反向筛查%梅毒螺旋体酶联免疫吸附试验%快速血浆反应素试验%梅毒螺旋体明胶颗粒凝集试验
梅毒%反嚮篩查%梅毒螺鏇體酶聯免疫吸附試驗%快速血漿反應素試驗%梅毒螺鏇體明膠顆粒凝集試驗
매독%반향사사%매독라선체매련면역흡부시험%쾌속혈장반응소시험%매독라선체명효과립응집시험
Syphilis%Reverse screening%ELISA%RPR%TPPA
目的：分析梅毒螺旋体酶联免疫吸附试验（TP - ELISA），梅毒螺旋体明胶颗粒凝集试验（TPPA），快速血浆反应素试验（RPR）三种常用梅毒筛查试验的特点，选择可靠的梅毒反向筛查流程，以提高检测准确性和筛查效率，避免漏检、误诊。方法回顾性分析11673例梅毒血清抗体检测标本的 TP - ELISA、RPR 和 TPPA 的检测数据。结果RPR 的特异度为99.9%，灵敏度为33.6%，假阴率66.4%，假阳性率0.052%，总符合率为99.1%，阳性率0.48%与 TP- ELISA 和 TPPA 比较均有显著性差异（ P ﹤0.05）。TP - ELISA 的特异度为99.8%，灵敏度为100%，假阴性率0，假阳性率0.156%，总符合率为99.8%，阳性率1.43%与 TPPA 比较无显著性差异（ P ﹥0.05），RPR 和 TP - ELISA 分别与TPPA 进行一致性比较，Kappa 系数为0.484和0.942。结论 ELISA 灵敏度高，适合于初筛；TPPA 特异度好，适合于初筛后的确认试验；RPR 仅适合于确认试验后梅毒治疗滴度检查及疗效监测。
目的：分析梅毒螺鏇體酶聯免疫吸附試驗（TP - ELISA），梅毒螺鏇體明膠顆粒凝集試驗（TPPA），快速血漿反應素試驗（RPR）三種常用梅毒篩查試驗的特點，選擇可靠的梅毒反嚮篩查流程，以提高檢測準確性和篩查效率，避免漏檢、誤診。方法迴顧性分析11673例梅毒血清抗體檢測標本的 TP - ELISA、RPR 和 TPPA 的檢測數據。結果RPR 的特異度為99.9%，靈敏度為33.6%，假陰率66.4%，假暘性率0.052%，總符閤率為99.1%，暘性率0.48%與 TP- ELISA 和 TPPA 比較均有顯著性差異（ P ﹤0.05）。TP - ELISA 的特異度為99.8%，靈敏度為100%，假陰性率0，假暘性率0.156%，總符閤率為99.8%，暘性率1.43%與 TPPA 比較無顯著性差異（ P ﹥0.05），RPR 和 TP - ELISA 分彆與TPPA 進行一緻性比較，Kappa 繫數為0.484和0.942。結論 ELISA 靈敏度高，適閤于初篩；TPPA 特異度好，適閤于初篩後的確認試驗；RPR 僅適閤于確認試驗後梅毒治療滴度檢查及療效鑑測。
목적：분석매독라선체매련면역흡부시험（TP - ELISA），매독라선체명효과립응집시험（TPPA），쾌속혈장반응소시험（RPR）삼충상용매독사사시험적특점，선택가고적매독반향사사류정，이제고검측준학성화사사효솔，피면루검、오진。방법회고성분석11673례매독혈청항체검측표본적 TP - ELISA、RPR 화 TPPA 적검측수거。결과RPR 적특이도위99.9%，령민도위33.6%，가음솔66.4%，가양성솔0.052%，총부합솔위99.1%，양성솔0.48%여 TP- ELISA 화 TPPA 비교균유현저성차이（ P ﹤0.05）。TP - ELISA 적특이도위99.8%，령민도위100%，가음성솔0，가양성솔0.156%，총부합솔위99.8%，양성솔1.43%여 TPPA 비교무현저성차이（ P ﹥0.05），RPR 화 TP - ELISA 분별여TPPA 진행일치성비교，Kappa 계수위0.484화0.942。결론 ELISA 령민도고，괄합우초사；TPPA 특이도호，괄합우초사후적학인시험；RPR 부괄합우학인시험후매독치료적도검사급료효감측。
Objective To investigate TP - enzyme - linked immunosorbent assay(ELISA),rapid plasma reagin test(RPR),trepone-ma pallidum gelatin particle agglutination test(TPPA)the characteristics of three kinds of commonly used test and select reliable reverse syphilis screening process,in order to improve the detection accuracy and screening efficiency,avoid leak and misdiagnosis. Methods Retrospective de-tection data analysis was performed in TP - ELISA,RPR,TPPA test in 11 673 cases of patients. Results The specific degree of the RPR was 99. 9% ,sensitivity was 33. 6% ,false negative rate was 66. 4% ,false - positive rate was 0. 052% ,the total coincidence rate was 99. 1% ,posi-tive rate was 0. 48% ,which had had significant difference compared with TP - ELISA and TPPA( P ﹤ 0. 05). The specific degree of the ELISA was 99. 8% ,sensitivity was 100% ,false negative rate was 0% ,false positive rate was 0. 156% ,the total coincidence rate was 99. 8% ,positive rate was 1. 43% ,which did not show significant difference compared with TPPA( P ﹥ 0. 05). RPR and TP - ELISA and TPPA are compared re-spectively,and the consistency Kappa coefficient was 0. 484 and 0. 942. Conclusion ELISA had the advantages of high sensitivity,be suitable for screening. The specificity of TPPA was good,be suitable for confirmation test after the screening. RPR was only suitable for confirmation test after inspection and monitoring the efficacy of treatment of syphilis titer.
