中国医药导报
中國醫藥導報
중국의약도보
CHINA MEDICAL HERALD
2014年
25期
22-25
,共4页
桑叶%肿瘤血管%内皮细胞%增殖%迁移%管腔形成
桑葉%腫瘤血管%內皮細胞%增殖%遷移%管腔形成
상협%종류혈관%내피세포%증식%천이%관강형성
Mulberry leaf%Tumor angiogenesis%Endothelial cells%Proliferation%Migration%Lumen formation
目的：研究桑叶对肿瘤血管生成中血管内皮细胞增殖、迁移和管腔形成的影响。方法用CCK-8试剂盒测定HUVEC-2C细胞数量的变化；用Transwell转移小室研究HUVEC-2C细胞迁移情况；用包被Matrigel的24孔板来培养HUVEC-2C细胞观察其管腔形成情况。实验分为实验组和对照组，每组分别设置3个复孔，在实验组中加入桑叶浸出液。观察桑叶对肿瘤血管生成的影响。结果桑叶液可抑制HUVEC-2C细胞的增殖能力，抑制作用随着浓度的增长而增强。在浓度为100、50、25 mg/mL时，与对照组比较，差异有统计学意义(P<0.05)。当浓度为100 mg/mL的桑叶液时细胞增殖抑制率可达到37.6%。通过观察Transwell小室，与对照组比较，添加了桑叶液的HUVEC-2C细胞，其迁移能力受到一定的制约作用。Matrigel实验中，与未加入桑叶液的HUVEC-2C细胞所形成的较完整的环状结构比较，加入桑叶液的HUVEC-2C细胞在管腔形成方面受到明显的抑制作用。结论桑叶液通过抑制血管内皮细胞增殖、迁移和管腔形成，对肿瘤血管的生成具有抑制作用。
目的：研究桑葉對腫瘤血管生成中血管內皮細胞增殖、遷移和管腔形成的影響。方法用CCK-8試劑盒測定HUVEC-2C細胞數量的變化；用Transwell轉移小室研究HUVEC-2C細胞遷移情況；用包被Matrigel的24孔闆來培養HUVEC-2C細胞觀察其管腔形成情況。實驗分為實驗組和對照組，每組分彆設置3箇複孔，在實驗組中加入桑葉浸齣液。觀察桑葉對腫瘤血管生成的影響。結果桑葉液可抑製HUVEC-2C細胞的增殖能力，抑製作用隨著濃度的增長而增彊。在濃度為100、50、25 mg/mL時，與對照組比較，差異有統計學意義(P<0.05)。噹濃度為100 mg/mL的桑葉液時細胞增殖抑製率可達到37.6%。通過觀察Transwell小室，與對照組比較，添加瞭桑葉液的HUVEC-2C細胞，其遷移能力受到一定的製約作用。Matrigel實驗中，與未加入桑葉液的HUVEC-2C細胞所形成的較完整的環狀結構比較，加入桑葉液的HUVEC-2C細胞在管腔形成方麵受到明顯的抑製作用。結論桑葉液通過抑製血管內皮細胞增殖、遷移和管腔形成，對腫瘤血管的生成具有抑製作用。
목적：연구상협대종류혈관생성중혈관내피세포증식、천이화관강형성적영향。방법용CCK-8시제합측정HUVEC-2C세포수량적변화；용Transwell전이소실연구HUVEC-2C세포천이정황；용포피Matrigel적24공판래배양HUVEC-2C세포관찰기관강형성정황。실험분위실험조화대조조，매조분별설치3개복공，재실험조중가입상협침출액。관찰상협대종류혈관생성적영향。결과상협액가억제HUVEC-2C세포적증식능력，억제작용수착농도적증장이증강。재농도위100、50、25 mg/mL시，여대조조비교，차이유통계학의의(P<0.05)。당농도위100 mg/mL적상협액시세포증식억제솔가체도37.6%。통과관찰Transwell소실，여대조조비교，첨가료상협액적HUVEC-2C세포，기천이능력수도일정적제약작용。Matrigel실험중，여미가입상협액적HUVEC-2C세포소형성적교완정적배상결구비교，가입상협액적HUVEC-2C세포재관강형성방면수도명현적억제작용。결론상협액통과억제혈관내피세포증식、천이화관강형성，대종류혈관적생성구유억제작용。
Objective To study the influence of mulberry on vascular endothelial cell proliferation and migration as well as lumen forming in tumor angiogenesis. Methods The change of HUVEC-2C cell quantity was measured with CCK-8 kit; HUVEC-2C cell migration was studied in Transwell chamber; HUVEC-2C cells were cultured in a 24-pore plate coated with Matrigel to observe its lumen forming. The experiment consisted of experiment group and control group, with three complex holes set for each group. Mulberry leachate was added to the experiment group. The effect of mulberry leaf to tumor angiogenesis was observed. Results Mulberry leachate could inhibit the proliferation ability of HUVEC-2C cell, with the inhibiting effect strengthened as the concentration increases. When the concentration was 100, 50, 25 mg/mL, the difference between the experiment group and the control group had statistical significance (P<0.05). When the concentration was 100 mg/mL, the cell proliferation inhibiting ratio of mulberry leachate could reach 37.6%. Observation on Transwell chamber showed the follows:added with mulberry leachate, the HUVEC-2C cells’mi-gration ability was inhibited to some extent compared with the control group. In Matrigel experiment, compared with the comparatively complete cyclic structure formed by HUVEC-2C cells without mulberry leachate, the HUVEC-2C cells added with mulberry leachate were obviously inhibited in term of lumen forming. Conclusion Mulberry leachate has inhibiting effect on tumor angiogenesis by inhibiting vascular endothelial cell proliferation and migration as well as lu-men forming.
