CAJ | 학술논문

目的：研究柴胡注射液和丹参注射液在犬肾近端小管上皮细胞(MDCK细胞)和人胚肾细胞(HEK-293细胞)模型的毒性作用，并探讨中药注射液体外肾细胞毒性检测的实验方法。方法选用马兜铃酸A作为阳性对照，将不同厂家和批次的丹参注射液、柴胡注射液的原液及稀释液，与MDCK细胞或HEK-293细胞共同孵育，通过四甲基偶氮唑盐比色法(MTT法)量化细胞毒性，计算相对增殖率，进行中药注射液体外肾细胞毒性评价。结果在MDCK细胞模型中，两批丹参注射4个液稀释浓度下毒性分级显示为0级，一批稀释9、27、81倍后显示为0级，柴胡注射液稀释27、81倍后显示为0级；在HEK-293细胞模型中，一批丹参注射液4个稀释浓度下毒性分级显示为1级，一批稀释9、27、81倍后显示为1级，一批稀释27、81倍后显示为1级；柴胡注射液稀释27、81倍后显示为2级。结论利用肾脏细胞来源的细胞，可尝试建立中药注射液体外肾细胞毒性检测的实验方法。
목적：연구시호주사액화단삼주사액재견신근단소관상피세포(MDCK세포)화인배신세포(HEK-293세포)모형적독성작용，병탐토중약주사액체외신세포독성검측적실험방법。방법선용마두령산A작위양성대조，장불동엄가화비차적단삼주사액、시호주사액적원액급희석액，여MDCK세포혹HEK-293세포공동부육，통과사갑기우담서염비색법(MTT법)양화세포독성，계산상대증식솔，진행중약주사액체외신세포독성평개。결과재MDCK세포모형중，량비단삼주사4개액희석농도하독성분급현시위0급，일비희석9、27、81배후현시위0급，시호주사액희석27、81배후현시위0급；재HEK-293세포모형중，일비단삼주사액4개희석농도하독성분급현시위1급，일비희석9、27、81배후현시위1급，일비희석27、81배후현시위1급；시호주사액희석27、81배후현시위2급。결론이용신장세포래원적세포，가상시건립중약주사액체외신세포독성검측적실험방법。
Objective To study the cytotoxicity of Salvia Injection and Bupleurum Injection in MDCK cells and HEK-293 cells and discuss the applicability of these experimental methods. Methods Aristolochic acid A was selected as a positive control drug. MDCK cells and HEK-293 cells were incubated with different concentrations of Salvia Injection or Bupleurum Injection. Then cytotoxicity was evaluated with MTT assay and relative growth rate (RGR) was calculated. Results In MDCK cell model, the RGR from two batches of Salvia Injection were shown as Class 0 after diluting 4 lev-els and one was shown as Class 0 after diluting 9, 27, 81 times. The RGR of Bupleurum Injection was shown as Class 0 after diluting 27, 81 times. Meanwhile, in HEK-293 cell model, the RGR from one batch of Salvia Injection was shown as Class 1 after diluting 4 levels. One batch of Salvia Injection was shown as Class 1 after diluting 9, 27, 81 times. One was shown as Class 1 after diluting 27, 81 times. The RGR of Bupleurum Injection was shown as Class 2 after diluting 27, 81 times. Conclusion The use of kidney-derived cells can help to establish experimental methods to test the Chi-nese medicine injection nephrotoxicity.