山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
33期
1-3,6
,共4页
肺腺癌%骨桥蛋白%CD44 v6
肺腺癌%骨橋蛋白%CD44 v6
폐선암%골교단백%CD44 v6
lung adenocarcinoma%OPN%CD44v6
目的：探讨骨桥蛋白（ OPN）及其受体CD44v6在肺腺癌侵袭转移中的作用。方法应用免疫组化方法检测129例癌旁组织、肺原位腺癌（AIS）、伏壁样生长为主型腺癌（LPA）患者手术切除标本中OPN、CD44v6的表达情况。检测H358细胞（原位癌细胞系）和A549细胞中OPN、CD44v6蛋白及mRNA的表达，Transwell 试验观察OPN、CD44 v6对H358细胞、A549细胞侵袭力的影响。结果 OPN、CD44 v6在LPA组织中的表达阳性率显著高于AIS及癌旁组织，AIS高于癌旁组织，OPN与CD44v6的表达呈显著正相关（ P＜0．05）。 OPN、CD44v6及OPN mR-NA、CD44v6 mRNA在A549细胞中的表达水平高于H358细胞。 Transwell试验提示OPN对A549细胞的趋化作用高于H358细胞。使用OPN抗体阻断OPN对两种细胞的趋化作用，两种细胞穿过数均减少；阻断CD44 v6受体通道后，OPN对两种细胞的趋化作用明显减弱。结论 OPN-CD44 v6复合体对肺腺癌的侵袭能力具有重要作用，OPN有望成为评估肺腺癌进展及预测肿瘤转移潜能的指标。
目的：探討骨橋蛋白（ OPN）及其受體CD44v6在肺腺癌侵襲轉移中的作用。方法應用免疫組化方法檢測129例癌徬組織、肺原位腺癌（AIS）、伏壁樣生長為主型腺癌（LPA）患者手術切除標本中OPN、CD44v6的錶達情況。檢測H358細胞（原位癌細胞繫）和A549細胞中OPN、CD44v6蛋白及mRNA的錶達，Transwell 試驗觀察OPN、CD44 v6對H358細胞、A549細胞侵襲力的影響。結果 OPN、CD44 v6在LPA組織中的錶達暘性率顯著高于AIS及癌徬組織，AIS高于癌徬組織，OPN與CD44v6的錶達呈顯著正相關（ P＜0．05）。 OPN、CD44v6及OPN mR-NA、CD44v6 mRNA在A549細胞中的錶達水平高于H358細胞。 Transwell試驗提示OPN對A549細胞的趨化作用高于H358細胞。使用OPN抗體阻斷OPN對兩種細胞的趨化作用，兩種細胞穿過數均減少；阻斷CD44 v6受體通道後，OPN對兩種細胞的趨化作用明顯減弱。結論 OPN-CD44 v6複閤體對肺腺癌的侵襲能力具有重要作用，OPN有望成為評估肺腺癌進展及預測腫瘤轉移潛能的指標。
목적：탐토골교단백（ OPN）급기수체CD44v6재폐선암침습전이중적작용。방법응용면역조화방법검측129례암방조직、폐원위선암（AIS）、복벽양생장위주형선암（LPA）환자수술절제표본중OPN、CD44v6적표체정황。검측H358세포（원위암세포계）화A549세포중OPN、CD44v6단백급mRNA적표체，Transwell 시험관찰OPN、CD44 v6대H358세포、A549세포침습력적영향。결과 OPN、CD44 v6재LPA조직중적표체양성솔현저고우AIS급암방조직，AIS고우암방조직，OPN여CD44v6적표체정현저정상관（ P＜0．05）。 OPN、CD44v6급OPN mR-NA、CD44v6 mRNA재A549세포중적표체수평고우H358세포。 Transwell시험제시OPN대A549세포적추화작용고우H358세포。사용OPN항체조단OPN대량충세포적추화작용，량충세포천과수균감소；조단CD44 v6수체통도후，OPN대량충세포적추화작용명현감약。결론 OPN-CD44 v6복합체대폐선암적침습능력구유중요작용，OPN유망성위평고폐선암진전급예측종류전이잠능적지표。
Objective To investigate the role of OPN and CD44v6 in the progression and invasion of lung adenocarci-noma.Methods OPN and CD44v6 expressions were detected in 129 patients with pericarcinomatous tissue , lung adeno-carcinoma in situ ( AIS ) and lepidic predominant adenocarcinoma ( LPA ) by immunohistochemical method .OPN and CD44v6 expressions were detected in H358 cells (lung adenocarcinoma in situ cell line ) and A549 cells by Western blot. Quantitative PCR was used to determine the expression levels of OPN mRNA and CD 44v6 mRNA.The influence of OPN and CD44 v6 on the invasive ability of H 358 cells and A549 cells was examined by Transwell chamber assay .Results Im-munohistochemical method revealed that the positive expression rates of OPN and CD 44v6 in LPA were significantly higher than those in AIS and pericarcinomatous tissue;the positive rates were higher in AIS compared to pericarcinomatous tissue . The expressions of these two markers were correlated positively (P<0.05).Western blot revealed that the positive expres-sion rates of OPN and CD44v6 in A549 cells were higher than those in H358 cells.Quantitative PCR revealed that the ex-pression levels of OPN and CD44v6 in A549 cells were higher than those in H358 cells.Transwell chamber assay revealed that OPN induced chemotaxis of cancer cells , and the chemotaxis was higher in A 549 cells than that in H358 cells.The mi-gration cell numbers of the two kinds of cancer cells decreased when blocked by OPN antibody .When the CD44 v6 receptor channel was blocked , OPN's chemotactic effect was weakened .Conclusion The OPN-CD44v6 complex plays an important role in lung adenocarcinoma progression;OPN might be used as an indicator to predict the progression and metastatic poten-tial of lung adenocarcinoma .
