CAJ | 학술논문

背景：课题组的前期工作主要围绕孤束核在接受胃伤害性刺激和针刺穴位反应中所扮演的角色展开研究，而迷走神经背核与孤束核、最后区一起作为迷走神经复合体中不可或缺的组成部分，在调节胃功能中发挥了重要的作用。目的：观察针刺“足三里”、“内关”、“脾俞”、“肾俞”等不同神经节段穴位对胃扩张模型大鼠迷走神经背核胃相关神经元的影响。方法：在成功构建大鼠胃扩张造模的基础上，采用神经电生理学的方法，运用细胞外记录技术，找出迷走神经背核胃相关神经元，予以手针刺激“足三里”、“内关”、“脾俞”、“肾俞”等不同神经节段穴位各15 s，记录来自体表的刺激对迷走神经背核胃相关神经元的影响。结果与结论：24只大鼠共记录到90个神经元放电，90个下丘脑室旁核神经元中与胃扩张相关的神经元有54个，54个胃扩张相关神经元中，对针刺足三里、内关、脾俞、肾俞有反应的神经元个数分别为44，39，38，27，有反应的神经元出现比例分别为82%，72%，70%，50%。提示针刺不同神经节段穴位均能不同程度地激活迷走神经背核参与调节胃运动。
배경：과제조적전기공작주요위요고속핵재접수위상해성자격화침자혈위반응중소분연적각색전개연구，이미주신경배핵여고속핵、최후구일기작위미주신경복합체중불가혹결적조성부분，재조절위공능중발휘료중요적작용。목적：관찰침자“족삼리”、“내관”、“비유”、“신유”등불동신경절단혈위대위확장모형대서미주신경배핵위상관신경원적영향。방법：재성공구건대서위확장조모적기출상，채용신경전생이학적방법，운용세포외기록기술，조출미주신경배핵위상관신경원，여이수침자격“족삼리”、“내관”、“비유”、“신유”등불동신경절단혈위각15 s，기록래자체표적자격대미주신경배핵위상관신경원적영향。결과여결론：24지대서공기록도90개신경원방전，90개하구뇌실방핵신경원중여위확장상관적신경원유54개，54개위확장상관신경원중，대침자족삼리、내관、비유、신유유반응적신경원개수분별위44，39，38，27，유반응적신경원출현비례분별위82%，72%，70%，50%。제시침자불동신경절단혈위균능불동정도지격활미주신경배핵삼여조절위운동。
BACKGROUND:Preliminary studies of our research group mainly focus on the role of nucleus of solitary tract in gastric noxious stimulation and acupuncture point, while dorsal nucleus of vagus nerve, nucleus of solitary tract and area postrema are the necessary component for vagus nerve complex, and dorsal nucleus of vagus nerve plays a crucial role in the regulation of gastric functions. OBJECTIVE:To observe the effect of acupuncture at Zusanli, Neiguan, Pishu and Shenshu acupoints on the gastric-related neurons in dorsal motor nucleus of the vagus nerve. METHODS:The gastric related neurons in dorsal motor nucleus of the vagus nerve of gastric distension rats were identified using extracellular recording technique, according to neuroelectrophysiology method. Acupuncture stimulation was given to Zusanli, Neiguan, Pishu and Shenshu for 15 seconds. The effect of body stimulation on the gastric distension related neurons in dorsal motor nucleus of the vagus nerve of rats was recorded. RESULTS AND CONCLUSION:Among the involved 24 rats, 90 neurons at paraventricular hypothalamic necleus exhibited discharge. Among the discharged neurons, 54 neurons were related to gastric distension. After acupuncture stimulation at Zusanli, Neiguan, Pishu and Shenshu, there were 44, 39, 38, 27 neurons giving a response, the reaction rate was 82%, 72%, 70%and 50%, respectively. Acupunture at different nerve segment acupoints can activate the dorsal motor nucleus of the vagus nerve to different degrees, which is involved in the regulation of gastric motility.