中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
36期
5830-5835
,共6页
实验动物%组织构建%补阳还五汤%断肢再植%肢体%缺血再灌注
實驗動物%組織構建%補暘還五湯%斷肢再植%肢體%缺血再灌註
실험동물%조직구건%보양환오탕%단지재식%지체%결혈재관주
drugs,Chinese herbal%reperfusion injury%muscle,skeletal
背景：近年来，中医药在缺血再灌注损伤的临床诊疗中发挥着独特作用。目的：探讨补阳还五汤在大鼠下肢断肢再植后缺血再灌注损伤中的影响，评估其对缺血组织的保护作用及机制。方法：将120只Wistar大鼠随机均分为4组，正常对照组仅麻醉及灌胃给予生理盐水，其余3组建立下肢断肢再植后缺血再灌注损伤动物模型，补阳还五汤组再灌注即刻灌胃给予补阳还五汤10 mL/kg，依达拉奉组再灌注即刻尾静脉注射依达拉奉10 mL/kg，空白对照组灌胃给予生理盐水。再灌注24 h后，测定血液髓过氧化酶、乳酸脱氢酶、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶水平；再灌注1周后，检测腓肠肌组织中超氧化物歧化酶活性、一氧化氮及丙二浓度、钙离子负载，采用RT-PCR半定量分析腓肠肌组织中bcl-2 mRNA和bax mRNA转录水平。结果与结论：与空白对照组、依达拉奉组相比，补阳还五汤可有效降低肢体缺血-再灌注后氧自由基的产生，增强超氧化物歧化酶活性，减轻钙超载，减少血浆一氧化氮的大量生成，增强抗凋亡基因bcl-2的表达，减弱促进凋亡基因bax的表达，减轻肢体缺血再灌注后骨骼肌损伤程度。结果表明补阳还五汤对大鼠断肢再植后下肢缺血再灌注损伤具有明显的保护作用，能有效清除自由基，抑制骨骼肌细胞凋亡，减轻再灌注损伤。
揹景：近年來，中醫藥在缺血再灌註損傷的臨床診療中髮揮著獨特作用。目的：探討補暘還五湯在大鼠下肢斷肢再植後缺血再灌註損傷中的影響，評估其對缺血組織的保護作用及機製。方法：將120隻Wistar大鼠隨機均分為4組，正常對照組僅痳醉及灌胃給予生理鹽水，其餘3組建立下肢斷肢再植後缺血再灌註損傷動物模型，補暘還五湯組再灌註即刻灌胃給予補暘還五湯10 mL/kg，依達拉奉組再灌註即刻尾靜脈註射依達拉奉10 mL/kg，空白對照組灌胃給予生理鹽水。再灌註24 h後，測定血液髓過氧化酶、乳痠脫氫酶、總一氧化氮閤酶、誘導型一氧化氮閤酶、結構型一氧化氮閤酶水平；再灌註1週後，檢測腓腸肌組織中超氧化物歧化酶活性、一氧化氮及丙二濃度、鈣離子負載，採用RT-PCR半定量分析腓腸肌組織中bcl-2 mRNA和bax mRNA轉錄水平。結果與結論：與空白對照組、依達拉奉組相比，補暘還五湯可有效降低肢體缺血-再灌註後氧自由基的產生，增彊超氧化物歧化酶活性，減輕鈣超載，減少血漿一氧化氮的大量生成，增彊抗凋亡基因bcl-2的錶達，減弱促進凋亡基因bax的錶達，減輕肢體缺血再灌註後骨骼肌損傷程度。結果錶明補暘還五湯對大鼠斷肢再植後下肢缺血再灌註損傷具有明顯的保護作用，能有效清除自由基，抑製骨骼肌細胞凋亡，減輕再灌註損傷。
배경：근년래，중의약재결혈재관주손상적림상진료중발휘착독특작용。목적：탐토보양환오탕재대서하지단지재식후결혈재관주손상중적영향，평고기대결혈조직적보호작용급궤제。방법：장120지Wistar대서수궤균분위4조，정상대조조부마취급관위급여생리염수，기여3조건립하지단지재식후결혈재관주손상동물모형，보양환오탕조재관주즉각관위급여보양환오탕10 mL/kg，의체랍봉조재관주즉각미정맥주사의체랍봉10 mL/kg，공백대조조관위급여생리염수。재관주24 h후，측정혈액수과양화매、유산탈경매、총일양화담합매、유도형일양화담합매、결구형일양화담합매수평；재관주1주후，검측비장기조직중초양화물기화매활성、일양화담급병이농도、개리자부재，채용RT-PCR반정량분석비장기조직중bcl-2 mRNA화bax mRNA전록수평。결과여결론：여공백대조조、의체랍봉조상비，보양환오탕가유효강저지체결혈-재관주후양자유기적산생，증강초양화물기화매활성，감경개초재，감소혈장일양화담적대량생성，증강항조망기인bcl-2적표체，감약촉진조망기인bax적표체，감경지체결혈재관주후골격기손상정도。결과표명보양환오탕대대서단지재식후하지결혈재관주손상구유명현적보호작용，능유효청제자유기，억제골격기세포조망，감경재관주손상。
BACKGROUND:Traditional Chinese medicine plays a specific role in the clinical diagnosis and treatment of ischemia reperfusion injury. OBJECTIVE:To investigate the effect of Bu Yang Huan Wu Decoction on ischemia reperfusion injury in rats after replantation of of lower limbs, and evaluate the protective effect and mechanism of ischemic tissue. METHODS:A total of 120 Wistar rats were randomly divided into four groups:normal control (intragastric administration of saline after anesthesia), Bu Yang Huan Wu Decoction (intragastric administration of 10 mL/kg Bu Yang Huan Wu Decoction immediately after reperfusion), edaravone (intravenous administration of 10 mL/kg edaravone immediately after reperfusion), and blank control (intragastric administration of saline) groups. At 24 hours after reperfusion, the myeloperoxidase, lactate dehydrogenase, nitricoxide synthase, inducible nitricoxide synthase, constitutive nitricoxide synthase level in the blood were detected. At 1 week after reperfusion, the activity of superoxide dismutase in gastrocnemius tissue, nitrogen monoxidum and malondialdehyde, and the load of Ca2+were assayed. RT-PCR semiquantitative analysis was performed to detect bcl-2 mRNA and bax mRNA transcriptional levels in the gastrocnemius.RESULTS AND CONCLUSION:Bu Yang Huan Wu Decoction protected the rats from ischemia reperfusion injury by reducing the production of oxygen free radicals, reinforcing the activity of superoxide dismutase, lessening the calcium overload, decreasing the production of nitrogen monoxidum, up-regulating the expression of anti-apoptotic gene bcl-2, down-regulating the expression of pro-apoptotic gene bax, and attenuating the injury of skeletal muscle after ischemia reperfusion injury. Bu Yang Huan Wu Decoction has significant protection on cerebral ischemia reperfusion injury in rats after replantation of severed limb, which may be related to the scavenge of free radicals, inhibition of skeletal cells apoptosis, and attenuation of reperfusion injury.
