CAJ | 학술논문

背景：减少体外模拟体内生理环境寄养断肢系统的缺血再灌注损伤，可以延长断肢的保存时间，提高断肢的再植成功率。目的：观察脉络宁对体外模拟体内生理环境寄养断肢系统缺血再灌注损伤的影响。方法：健康成年雄性巴马小型猪18只，随机分成冷藏组、血液灌注组和脉络宁联合血液灌注组，每组6只。将每只猪的左后肢离断室温保存3h后，冷藏组置于4℃冷藏，血液灌注组用血液灌注、脉络宁联合血液灌注组用脉络宁联合血液灌注。灌注6h后，采用透射电镜分别观察各组离断肢体骨骼肌形态学变化；实时定量RT-PCR检测离断肢体骨骼肌Caspase3和白细胞介素1βmRNA表达。结果与结论：透射电镜结果显示，血液灌注组、脉络宁联合血液灌注组均较冷藏组肌纤维整齐、肌节完整、线粒体肿胀程度轻，且脉络宁联合血液灌注组情况优于血液灌注组；实时定量RT-PCR检测结果显示，3组的caspase3和白细胞介素1β的表达均高于正常，同时血液灌注组和脉络宁联合血液灌注组的caspase3和白细胞介素1β的表达水平明显低于冷藏组，且脉络宁联合血液灌注组表达水平低于血液灌注组。结果提示在体外模拟体内生理环境寄养断肢系统中，脉络宁可显著减轻断肢骨骼肌细胞的缺血再灌注损伤，延长断肢的保存时间。
배경：감소체외모의체내생리배경기양단지계통적결혈재관주손상，가이연장단지적보존시간，제고단지적재식성공솔。목적：관찰맥락저대체외모의체내생리배경기양단지계통결혈재관주손상적영향。방법：건강성년웅성파마소형저18지，수궤분성랭장조、혈액관주조화맥락저연합혈액관주조，매조6지。장매지저적좌후지리단실온보존3h후，랭장조치우4℃랭장，혈액관주조용혈액관주、맥락저연합혈액관주조용맥락저연합혈액관주。관주6h후，채용투사전경분별관찰각조리단지체골격기형태학변화；실시정량RT-PCR검측리단지체골격기Caspase3화백세포개소1βmRNA표체。결과여결론：투사전경결과현시，혈액관주조、맥락저연합혈액관주조균교랭장조기섬유정제、기절완정、선립체종창정도경，차맥락저연합혈액관주조정황우우혈액관주조；실시정량RT-PCR검측결과현시，3조적caspase3화백세포개소1β적표체균고우정상，동시혈액관주조화맥락저연합혈액관주조적caspase3화백세포개소1β적표체수평명현저우랭장조，차맥락저연합혈액관주조표체수평저우혈액관주조。결과제시재체외모의체내생리배경기양단지계통중，맥락저가현저감경단지골격기세포적결혈재관주손상，연장단지적보존시간。
BACKGROUND:Reducing the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs, can extend the preservation time and improve the replantation success rate of amputated limb. OBJECTIVE:To investigate the effect of Mailuoning on the ischemia-reperfusion injury in the system of simulated in vivo physiological environment for amputated limbs. METHODS:The left hind legs harvested from 18 healthy adult male Bama mini-pigs were randomly divided into three groups:cold storage group, blood perfusion group, Mailuoning combined blood perfusion group, with six pigs in each group. After the left hind legs of each pig in al groups were amputated and stored at room temperature for 3 hours, the amputated legs were placed at 4 ℃, perfused with blood, or perfused with Mailuoning combined blood. After 6 hours of perfusion, the morphology of amputated limbs was observed under transmission electron microscope. The mRNA levels of apoptosis proteins Caspase3 and inflammatory factor interleukin-1βwere detected by real time fluorescent quantitative RT-PCR.RESULTS AND CONCLUSION:Transmission electron microscopy results showed that, the muscle fibers in blood perfusion group and Mailuoning combined blood perfusion group arranged more orderly and were more complete than cold storage group, the swel ing of mitochondria was lighter. In addition, the condition in Mailuoning combined blood perfusion group was better than in blood perfusion group. RT-PCR results showed that the expression of Caspase3 and interleukin-1βwas increased in al groups;at the same time, the expression level in blood perfusion group and Mailuoning combined blood perfusion group was significantly lower than those in cold storage group. Mailuoning combined blood perfusion group had s lower expression than blood perfusion group. In the system of simulated in vivo physiological environment for amputated limbs, Mailuoning can significantly reduce the ischemia/reperfusion injury of skeletal muscle cells and prolong the preservation time of amputated limbs.