中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
36期
5819-5824
,共6页
实验动物%组织构建%沙棘总黄酮%血管性痴呆%修复重建%移植%肿瘤坏死因子α%白细胞介素1β
實驗動物%組織構建%沙棘總黃酮%血管性癡呆%脩複重建%移植%腫瘤壞死因子α%白細胞介素1β
실험동물%조직구건%사극총황동%혈관성치태%수복중건%이식%종류배사인자α%백세포개소1β
hippophae%flavones%dementia,vascular
背景：研究表明沙棘所含沙棘总黄酮对免疫系统的多环节都具有不同程度的调节能力，但有关沙棘总黄酮对血管性痴呆炎症因子影响的研究还鲜有报道。目的：观察沙棘总黄酮对血管性痴呆模型大鼠血清肿瘤坏死因子α、白细胞介素1β水平的影响，探讨其对血管性痴呆的干预机制。方法：取Wistar大鼠72只，随机分为假手术组、模型组、对照组、沙棘总黄酮高剂量组、沙棘总黄酮中剂量组、沙棘总黄酮低剂量组，后5组采用改进的“血管阻断法”建立血管性痴呆模型，假手术组仅切开分离双侧颈总动脉而不结扎。造模后1 d，对照组灌胃给予银杏提取物48 mg/(kg?d)，沙棘总黄酮高、中、低剂量组分别灌胃给予沙棘总黄酮200，100，50 mg/(kg?d)，模型组与假手术组灌胃给予等量生理盐水，连续灌胃30 d后进行Morris水迷宫行为学测试，并检测血清中肿瘤坏死因子α、白细胞介素1β水平。结果与结论：与假手术组比较，模型组大鼠学习记忆能力下降，血清肿瘤坏死因子α、白细胞介素1β水平增加(P<0.05)；经银杏叶提取物、沙棘总黄酮干预后，大鼠学习记忆能力得到改善，血清肿瘤坏死因子α、白细胞介素1β水平下降(P <0.05)。表明沙棘总黄酮能减少血管性痴呆模型大鼠血清肿瘤坏死因子α、白细胞介素1β水平，这可能是其干预血管性痴呆的主要作用机制之一。
揹景：研究錶明沙棘所含沙棘總黃酮對免疫繫統的多環節都具有不同程度的調節能力，但有關沙棘總黃酮對血管性癡呆炎癥因子影響的研究還鮮有報道。目的：觀察沙棘總黃酮對血管性癡呆模型大鼠血清腫瘤壞死因子α、白細胞介素1β水平的影響，探討其對血管性癡呆的榦預機製。方法：取Wistar大鼠72隻，隨機分為假手術組、模型組、對照組、沙棘總黃酮高劑量組、沙棘總黃酮中劑量組、沙棘總黃酮低劑量組，後5組採用改進的“血管阻斷法”建立血管性癡呆模型，假手術組僅切開分離雙側頸總動脈而不結扎。造模後1 d，對照組灌胃給予銀杏提取物48 mg/(kg?d)，沙棘總黃酮高、中、低劑量組分彆灌胃給予沙棘總黃酮200，100，50 mg/(kg?d)，模型組與假手術組灌胃給予等量生理鹽水，連續灌胃30 d後進行Morris水迷宮行為學測試，併檢測血清中腫瘤壞死因子α、白細胞介素1β水平。結果與結論：與假手術組比較，模型組大鼠學習記憶能力下降，血清腫瘤壞死因子α、白細胞介素1β水平增加(P<0.05)；經銀杏葉提取物、沙棘總黃酮榦預後，大鼠學習記憶能力得到改善，血清腫瘤壞死因子α、白細胞介素1β水平下降(P <0.05)。錶明沙棘總黃酮能減少血管性癡呆模型大鼠血清腫瘤壞死因子α、白細胞介素1β水平，這可能是其榦預血管性癡呆的主要作用機製之一。
배경：연구표명사극소함사극총황동대면역계통적다배절도구유불동정도적조절능력，단유관사극총황동대혈관성치태염증인자영향적연구환선유보도。목적：관찰사극총황동대혈관성치태모형대서혈청종류배사인자α、백세포개소1β수평적영향，탐토기대혈관성치태적간예궤제。방법：취Wistar대서72지，수궤분위가수술조、모형조、대조조、사극총황동고제량조、사극총황동중제량조、사극총황동저제량조，후5조채용개진적“혈관조단법”건립혈관성치태모형，가수술조부절개분리쌍측경총동맥이불결찰。조모후1 d，대조조관위급여은행제취물48 mg/(kg?d)，사극총황동고、중、저제량조분별관위급여사극총황동200，100，50 mg/(kg?d)，모형조여가수술조관위급여등량생리염수，련속관위30 d후진행Morris수미궁행위학측시，병검측혈청중종류배사인자α、백세포개소1β수평。결과여결론：여가수술조비교，모형조대서학습기억능력하강，혈청종류배사인자α、백세포개소1β수평증가(P<0.05)；경은행협제취물、사극총황동간예후，대서학습기억능력득도개선，혈청종류배사인자α、백세포개소1β수평하강(P <0.05)。표명사극총황동능감소혈관성치태모형대서혈청종류배사인자α、백세포개소1β수평，저가능시기간예혈관성치태적주요작용궤제지일。
BACKGROUND:Total flavonoids of hippophae have different degrees of regulation effect on the immune system. However, there is no available evidence addressing the effect of total flavonoids of hippophae on vascular dementia inflammatory factor. OBJECTIVE:To observe the effect of total flavonoids of hippophae on serum tumor necrosis factor-αand interleukin-1βlevels in a rat model of vascular dementia, and to discuss its interventional mechanism for vascular dementia. METHODS:A total of 72 Wistar rats were randomly divided into six groups:sham operation, model, control, high-dose, medium-dose, and low-dose of total flavonoids of hippophae groups. Except for the sham operation group, rats in the other five groups were applied to establish vascular dementia models using the improved“vascular occlusion”method. Sham operation group was only subjected to the isolation of bilateral common carotid artery, without ligation. At 1 day after modeling, rats in control group was intragastrical y given Ginkgo biloba extracts 48 mg/kg?d, while rats in total flavonoids of hippophae groups were given 200, 100, 50 mg/kg?d of total flavonoids of hippophae. Model group and sham operation group received equal volume of saline. After 30 days of administration, rats were detected in Morris water maze. Serum tumor necrosis factor-αand interleukin-1βlevels of rats in different groups were detected after intervention. RESULTS AND CONCLUSION:Compared with sham operation group, the vascular dementia model rats had a disordered learning and memory ability, and increased levels of serum tumor necrosis factor-αand interleukin-1β(P<0.05). After intervention of Ginkgo biloba extracts and total flavonoids of hippophae, the learning and memory abilities were improved, and serum tumor necrosis factor-αand interleukin-1βlevels were decreased (P<0.05). Total flavonoids of hippophae can reduce serum tumor necrosis factor-αand interleukin-1βlevels in vascular dementia rats. This may be the main mechanism of treating vascular dementia.
