CAJ | 학술논문

背景：炎性细胞活化、氧自由基的产生是肺缺血再灌注损伤发生的重要因素。在常用的肺保护液中增加可能具有抑制炎症、抗氧化作用的药物，使保护液进行一定的改良，对肺缺血再灌注损伤的研究及保护移植肺的功能具有重要的意义。目的：探讨去氢骆驼蓬碱对犬肺缺血再灌注损伤的作用。方法：将12只健康杂交犬随机分为两组，每组6只。建立犬肺缺血再灌注损伤模型，采用顺灌方式行保护液肺灌注，实验组给予低钾右旋糖苷保护液+去氢骆驼蓬碱溶液灌注，对照组给予低钾右旋糖苷保护液。缺血2h后，恢复左肺循环，两组于再灌注后采集左肺组织及血液标本，检测细胞因子水平，计算肺湿/干质量比；收集支气管肺泡灌洗液，观察各项病理指标；连续测量记录主肺动脉压、左肺动脉压和右肺动脉压。结果与结论：两组于再灌注2，4 h时左肺组织及血液中的白细胞介素17、肿瘤坏死因子α及内皮素1水平差异均无显著性意义(P>0.05)，两组再灌注4 h后的支气管肺泡灌洗液中性粒细胞数目、淋巴细胞数目、肺泡损伤指数及血管壁水肿程度差异均无显著性意义(P >0.05)，两组肺湿/干质量比差异均无显著性意义(P >0.05)，经方差分析，两组主肺动脉压、左肺动脉压及右肺动脉压差异均无显著性意义(P>0.05)。结果可见通过对细胞因子、病理指标、肺组织湿/干质量比及肺动脉压力分析，去氢骆驼蓬碱在犬肺原位常温缺血再灌注损伤模型中没有明显的肺保护作用。
배경：염성세포활화、양자유기적산생시폐결혈재관주손상발생적중요인소。재상용적폐보호액중증가가능구유억제염증、항양화작용적약물，사보호액진행일정적개량，대폐결혈재관주손상적연구급보호이식폐적공능구유중요적의의。목적：탐토거경락타봉감대견폐결혈재관주손상적작용。방법：장12지건강잡교견수궤분위량조，매조6지。건립견폐결혈재관주손상모형，채용순관방식행보호액폐관주，실험조급여저갑우선당감보호액+거경락타봉감용액관주，대조조급여저갑우선당감보호액。결혈2h후，회복좌폐순배，량조우재관주후채집좌폐조직급혈액표본，검측세포인자수평，계산폐습/간질량비；수집지기관폐포관세액，관찰각항병리지표；련속측량기록주폐동맥압、좌폐동맥압화우폐동맥압。결과여결론：량조우재관주2，4 h시좌폐조직급혈액중적백세포개소17、종류배사인자α급내피소1수평차이균무현저성의의(P>0.05)，량조재관주4 h후적지기관폐포관세액중성립세포수목、림파세포수목、폐포손상지수급혈관벽수종정도차이균무현저성의의(P >0.05)，량조폐습/간질량비차이균무현저성의의(P >0.05)，경방차분석，량조주폐동맥압、좌폐동맥압급우폐동맥압차이균무현저성의의(P>0.05)。결과가견통과대세포인자、병리지표、폐조직습/간질량비급폐동맥압력분석，거경락타봉감재견폐원위상온결혈재관주손상모형중몰유명현적폐보호작용。
BACKGROUND:Inflammatory cellactivation and the generation of oxygen free radicals are important factors of lung ischemia-reperfusion injury. Adding the drugs with anti-inflammation and antioxidant effects into the lung preservation solution used, can improve the protection fluid, and play a crucial role in the study of lung ischemia-reperfusion injury and protection of the function of transplanted lung. OBJECTIVE:To discussion the effect of harmine in canine model of pulmonary ischemia-reperfusion injury. METHODS:Twelve healthy hybrid dogs were randomly divided into two groups, with six rats in each group. A canine model of lung ischemia-reperfusion injury was established, and the protecting liquid was perfused with the clockwise irrigation method. Control group:low potassium dextran protective fluid;experimental group:low potassium dextran+harmine protective fluid. After 2 hours of ischemia, the left lung circulation was recovered. The left lung tissue and blood samples were col ected from two groups of animal models after reperfusion, and their cytokines levels and the lung wet/dry weight ratio were detected and calculated. Bronchoalveolar lavage fluid was col ected to observe the pathological indicators. The main pulmonary artery pressure, left and right pulmonary artery pressure were recorded by continuous monitoring. RESULTS AND CONCLUSION:There were no statistical y significant differences in the interleukin-17, tumor necrosis factorαand endothelin 1 content in the left lung tissue and the blood between the two groups at 2 and 4 hours after reperfusion (P>0.05). After 4 hours of reperfusion in both groups, the neutrophil number, the number of lymphocytes, alveolar edema index, and vascular wal damage in bronchoalveolar lavage fluid showed no statistical y significant differences (P>0.05). Through the analysis of variance, the main pulmonary artery pressure, left pulmonary artery pressure and right pulmonary artery pressure also had no statistical significance between the two groups (P>0.05). By the analysis of cytokines, pathological indicators, lung wet/dry weight ratio, and pulmonary arterial pressure, harmine has no significant lung protection effect in canine model of lung ischemia-reperfusion injury.