CAJ | 학술논문

目的：考察超高效液相色谱（ UPLC）分析条件变化对汉黄芩苷转化因子（ K）测定的影响。方法配制含汉黄芩苷、汉黄芩素（摩尔浓度比为1∶1）一系列浓度的标准溶液，经UPLC定量测得两化合物峰面积，二者比值即K值。逐个改变分析条件研究K值变化情况。结果随流动相pH值由2．5逐渐增至3．5、4．5、6．5，测得K值升高10．7％～14．6％；pH值增至8．5时两化合物出峰时间明显前移，影响分析准确性。随流动相离子强度、样品介质pH值变化，K值变化很小（偏差＜4％）。检测波长由280 nm变至254、274、342 nm时，K值随之变化（4．1％～10．7％）。结论汉黄芩苷K值测定易受某些UPLC分析条件如流动相pH值、检测波长等变化的影响。对无对照品UGT代谢产物进行定量分析时，为保证结果准确可靠，在各种样品检测过程中最好保持分析条件完全一致。
목적：고찰초고효액상색보（ UPLC）분석조건변화대한황금감전화인자（ K）측정적영향。방법배제함한황금감、한황금소（마이농도비위1∶1）일계렬농도적표준용액，경UPLC정량측득량화합물봉면적，이자비치즉K치。축개개변분석조건연구K치변화정황。결과수류동상pH치유2．5축점증지3．5、4．5、6．5，측득K치승고10．7％～14．6％；pH치증지8．5시량화합물출봉시간명현전이，영향분석준학성。수류동상리자강도、양품개질pH치변화，K치변화흔소（편차＜4％）。검측파장유280 nm변지254、274、342 nm시，K치수지변화（4．1％～10．7％）。결론한황금감K치측정역수모사UPLC분석조건여류동상pH치、검측파장등변화적영향。대무대조품UGT대사산물진행정량분석시，위보증결과준학가고，재각충양품검측과정중최호보지분석조건완전일치。
Objective To investigate the effects of UPLC analysis conditions on the conversion factor ( K) determina-tion of wogonoside .Methods A series of standard working solutions containing wogonoside and wogonin ( molar ratio 1∶1 ) were prepared and determined by UPLC to obtain peak area ratio of the two compounds , which was equal to the value of K.Then, analysis conditions were altered respectively and observed the variance of K value .Results When pH value of mobile phase A rose from 2.5 to 3.5, 4.5 and 6.5, K values were found to increase 10.7%-14.6%.When the pH value rose to 8.5, the retention time of wogonoside and wogonin moved forward significantly , which affected the accuracy of K value determination .With the variance of ionic strength of mobile phase A and pH value of sample medium , the K values determined were similar (deviation<4%).With the variance of detection wavelength from predetermined 280 nm to 254, 274, 342 nm, deviation of K value was in the range of 4.1%-10.7%.Conclusions The K value determination of wogonoside is susceptible to the variance of several UPLC analysis conditions such as pH value of mobile phase A , detection wavelength .To provide more precise quanification for glucuronides which are usually not available commercially , it is nec-essary to keep UPLC analysis conditions consistent during the process of determination .