CAJ | 학술논문

目的：对毕赤酵母表达的 textlinin-1（Q8008）进行分离纯化，并对其抑制plasmin活性的性质进行研究。方法构建表达 Q8008 pachia pastoris工程菌，经发酵、纯化得到纯度达95％以上的 Q8008。考察不同的温度、pH 缓冲液和金属离子等条件对 Q8008抑制plasmin活性的影响。结果 Q8008在中性条件下比较稳定，其最适pH 值为7.0，最适温度为40℃，在30～50℃相对酶活力在90.0％以上；有2个金属离子对 Q8008活性测定方法的影响产生了显著性差异，Zn2＋起激活作用，Co2＋起抑制作用，其余金属离子均无显著性差异。结论 Q8008在中性 pH 下抑制活性稳定；在50℃以下，受温度变化影响较小；金属离子对其抑制活性测定有一定影响。
목적：대필적효모표체적 textlinin-1（Q8008）진행분리순화，병대기억제plasmin활성적성질진행연구。방법구건표체 Q8008 pachia pastoris공정균，경발효、순화득도순도체95％이상적 Q8008。고찰불동적온도、pH 완충액화금속리자등조건대 Q8008억제plasmin활성적영향。결과 Q8008재중성조건하비교은정，기최괄pH 치위7.0，최괄온도위40℃，재30～50℃상대매활력재90.0％이상；유2개금속리자대 Q8008활성측정방법적영향산생료현저성차이，Zn2＋기격활작용，Co2＋기억제작용，기여금속리자균무현저성차이。결론 Q8008재중성 pH 하억제활성은정；재50℃이하，수온도변화영향교소；금속리자대기억제활성측정유일정영향。
Objective The characteristics of Q8008 expressed in Pichia pastoris and purified from fermentation liquid were studied . Methods Measure the influence of temperature ,pH and metal ions on the inhibitory activity of Q8008 . Results The results show that the optimum pH is 7.0 .The optimum temperature is 40℃ .The activi-ties of the inhibitor remains 90.0% when temperature in the range 30~50℃ .Zn2+ ion could activate the activity of Q8008 ,while Co2+ ion have inhibitory action . Conclusion Q8008 is more stable at neutral pH ,and not very sen-sitive to changing tempreture 50℃ .Metal ions have certain influence on its inhibition activities determination .