食品工程
食品工程
식품공정
FOOD ENGINEERING
2014年
3期
55-57
,共3页
徐渊金%陈含笑%张婷%卜慧斐
徐淵金%陳含笑%張婷%蔔慧斐
서연금%진함소%장정%복혜비
反相高效液相色谱%糕点%防腐剂%甜味剂%添加剂
反相高效液相色譜%糕點%防腐劑%甜味劑%添加劑
반상고효액상색보%고점%방부제%첨미제%첨가제
RP-HPLC%cakes%preservative%sweetener%additive
建立反相高效液相色谱法(RP-HPLC)同时测定糕点中安赛蜜、苯甲酸、山梨酸、糖精钠和脱氢乙酸。该方法糕点样品经处理后,采用SunFire C18(5μm,4.6 mm×250 mm)反相色谱柱,流动相为甲醇:磷酸盐缓冲液(pH6.7)=7∶93等度洗脱,流速为1.0 mL/min,检测波长230 nm,柱温为30℃,进样量为20μL。线性范围0.2 mg/L~120 mg/L,检出限0.4 mg/kg~1.0 mg/kg,加标回收率为87.7%~105.3%,相对标准偏差小于5%。结果表明,该方法操作简便、结果可靠,可用于同时检测糕点中安赛蜜、苯甲酸、山梨酸、糖精钠和脱氢乙酸。
建立反相高效液相色譜法(RP-HPLC)同時測定糕點中安賽蜜、苯甲痠、山梨痠、糖精鈉和脫氫乙痠。該方法糕點樣品經處理後,採用SunFire C18(5μm,4.6 mm×250 mm)反相色譜柱,流動相為甲醇:燐痠鹽緩遲液(pH6.7)=7∶93等度洗脫,流速為1.0 mL/min,檢測波長230 nm,柱溫為30℃,進樣量為20μL。線性範圍0.2 mg/L~120 mg/L,檢齣限0.4 mg/kg~1.0 mg/kg,加標迴收率為87.7%~105.3%,相對標準偏差小于5%。結果錶明,該方法操作簡便、結果可靠,可用于同時檢測糕點中安賽蜜、苯甲痠、山梨痠、糖精鈉和脫氫乙痠。
건립반상고효액상색보법(RP-HPLC)동시측정고점중안새밀、분갑산、산리산、당정납화탈경을산。해방법고점양품경처리후,채용SunFire C18(5μm,4.6 mm×250 mm)반상색보주,류동상위갑순:린산염완충액(pH6.7)=7∶93등도세탈,류속위1.0 mL/min,검측파장230 nm,주온위30℃,진양량위20μL。선성범위0.2 mg/L~120 mg/L,검출한0.4 mg/kg~1.0 mg/kg,가표회수솔위87.7%~105.3%,상대표준편차소우5%。결과표명,해방법조작간편、결과가고,가용우동시검측고점중안새밀、분갑산、산리산、당정납화탈경을산。
RP-HPLC was applied to the separation and determination of acesulfame, benzoic acid, sorbic acid, sac-charin sodium, and dehydroacetic acid in cakes. SunFire C18 was used as reversed phase column, and a solution contain-ing methanol and phosphate buffered saline mixed in the ratio of 7 to 93 was used as the mobile phase. The flow was 1.0 mL/min, the detect wavelength was 230 nm, the column temperature was 30℃, and the injection volume was 20μL. The limit of detection was between 0.4 mg/kg and 1.0 mg/kg, the linear plots were obtained between 0.2 mg/L and 120 mg/L. The fortification recoveries were between 87.7% and 105.3%, and RSD (n=6) value were less than 5%. This method is simple and highly sensitive, can be used to determine acesulfame, benzoic acid, sorbic acid, saccharin sodium, and dehydroacetic acid in cakes simultaneously.