CAJ | 학술논문

本试验分别选用4种不同材质[尼龙66（NL 66）、聚醚砜（PES）、混合纤维素（MCE）和聚四氟乙烯（PTFE）]滤膜过滤茶样浸提液，考察微孔滤膜过滤对茶叶中没食子酸、儿茶素类和嘌呤碱HPLC分析检测的影响。结果表明同种材质滤膜连续过滤获得3瓶滤液（约1 mL/瓶）中的没食子酸和生物碱含量基本相当；尼龙66滤膜过滤液中的儿茶素含量存在较大的瓶间差异，但PES、MCE和PTFE滤膜过滤液并无明显的先后差别。由此可见，PES、MCE和PTFE等是茶样浸提液净化预处理较为适宜的滤膜材质，而吸附作用则可能是引起NL 66滤膜过滤液中儿茶素类含量测定结果不一致的主要原因。
본시험분별선용4충불동재질[니룡66（NL 66）、취미풍（PES）、혼합섬유소（MCE）화취사불을희（PTFE）]려막과려다양침제액，고찰미공려막과려대다협중몰식자산、인다소류화표령감HPLC분석검측적영향。결과표명동충재질려막련속과려획득3병려액（약1 mL/병）중적몰식자산화생물감함량기본상당；니룡66려막과려액중적인다소함량존재교대적병간차이，단PES、MCE화PTFE려막과려액병무명현적선후차별。유차가견，PES、MCE화PTFE등시다양침제액정화예처리교위괄의적려막재질，이흡부작용칙가능시인기NL 66려막과려액중인다소류함량측정결과불일치적주요원인。
In this experiment, effects of microfiltration on HPLC determination of catechins and alkaloids in tea were investigated with four different membrane materials, nylon 66 ( NL 66 ) , polyether sulfone ( PES ) , mixed cellulose ( MCE ) and polytetrafluoroethylene(PTFE). The results indicated that there was no obvious difference in the contents of gallic acid and alkaloids among three auto sampler vials(approximately 1mL per vial) when the tea infusions were continuously filtered with the same membrane. However, the contents of catechins in the filtrates obtained from NL 66 membrane filtration showed more difference between each vial and less difference would be found for MCE, PES and PTFE membrane filtration. These facts suggested that MCE, PES and PTFE filters were more suitable for pretreatment of tea infusions combined with HPLC determination of tea compositions, and the inaccuracy of HPLC determination of tea catechins with Nylon 66 filter might result from its absorption character.