CAJ | 학술논문

采用麦氏比浊法，将大肠埃希菌的菌悬液浓度调整到约8×108 cfu/ g，再将其稀释成多个梯度，在600 nm 的波长下用紫外分光光度计测定吸光度值，同时将各个梯度的菌悬液分别做菌落总数试验，得到不同吸光度值对应的菌落总数，并制作标准曲线，通过测量菌悬液的吸光度值确定菌悬液浓度及其适用范围。试验证明，用此法确定菌悬液浓度时，其菌落总数宜在105~108 cfu/ g 之间，不适用于在105 cfu/ g 以下的菌悬液，所得结果比麦氏比浊法准确。
채용맥씨비탁법，장대장애희균적균현액농도조정도약8×108 cfu/ g，재장기희석성다개제도，재600 nm 적파장하용자외분광광도계측정흡광도치，동시장각개제도적균현액분별주균락총수시험，득도불동흡광도치대응적균락총수，병제작표준곡선，통과측량균현액적흡광도치학정균현액농도급기괄용범위。시험증명，용차법학정균현액농도시，기균락총수의재105~108 cfu/ g 지간，불괄용우재105 cfu/ g 이하적균현액，소득결과비맥씨비탁법준학。
The McFarland’s turbidimetry method was used to adjust the concentration of E. coli bacterial suspension to 8 × 108 cfu/ g,then diluted into several gradients to measure absorbance values by UV spectrophotometer at 600 nm wavelength,at the same time,tested the total numbers of colony of several gradients by plate count method. Took total numbers of colony as X-axis and absorbance values as Y-axis to draw a standard curve. The purpose was to determine rapidly the concentration of bacterial suspension and the scope of application with the absorbance method. The result showed that the eligible concentration of bacterial suspension was 105 ~ 108 cfu/ g with the absorbance method,which could not be used for bacterial suspension below 105 cfu/ g,and the data were more accurate than those of McFarland’ s turbidimetry.