CAJ | 학술논문

从浙江省金华市郊水稻田土壤中筛到1株拮抗水稻白叶枯病菌的白蚁链霉菌（Streptomyces termitum） ACT-2菌株。该菌株的菌体和发酵液对水稻白叶枯病菌均表现出较强的拮抗能力；水稻盆栽生防试验结果表明：对水稻白叶枯病有较好的防效，生防效果最高可达80.80%。通过分步萃取、硅胶柱层析、凝胶柱层析等步骤，分离纯化获得拮抗活性物质，对目标物进行核磁共振氢谱分析和质谱分析，通过解析与比对该目标物为Aloesaponarin II（1-甲基-3，8-二羟基蒽醌），分子式为 C15 H10 O4。以氯霉素为对照，测定纯化的 Aloesaponarin II对水稻白叶枯病菌拮抗活性，结果表明 Aloesaponarin II 具有较强的抗菌活性，半抑制浓度为 IC50=19.2μg/mL，但较氯霉素弱（半抑制浓度为 IC50=0.39μg/ mL）。
종절강성금화시교수도전토양중사도1주길항수도백협고병균적백의련매균（Streptomyces termitum） ACT-2균주。해균주적균체화발효액대수도백협고병균균표현출교강적길항능력；수도분재생방시험결과표명：대수도백협고병유교호적방효，생방효과최고가체80.80%。통과분보췌취、규효주층석、응효주층석등보취，분리순화획득길항활성물질，대목표물진행핵자공진경보분석화질보분석，통과해석여비대해목표물위Aloesaponarin II（1-갑기-3，8-이간기은곤），분자식위 C15 H10 O4。이록매소위대조，측정순화적 Aloesaponarin II대수도백협고병균길항활성，결과표명 Aloesaponarin II 구유교강적항균활성，반억제농도위 IC50=19.2μg/mL，단교록매소약（반억제농도위 IC50=0.39μg/ mL）。
A Streptomyces termitum strain ACT-2 with antimicrobial activity against paddy white leaf wilt( Xan-thomonas oryzae pv. oryzae)(Xoo)race P6 was screened from rice field soil in the suburb of Jinhua. Thalamus of strain ACT-2 and its fermentation both possessed with fairly strong antimicrobial activity to Xoo race P6. The best bio-control effects of the fermentation broth of strain ACT-2 on Xoo in experiments in pot cultivated paddy reached as high as 80. 80% . In this study,step-by-step extraction,silica gel column chromatography,gel column chromatography, and other steps,and obtained an antagonistic active substance by isolation and purification. Structural determination was made by 1 H NMR and EI-MS analyses. The targeted substance was Aloesaponarin II(1-methyl-3,8-dihydroxyl-anthraquinone),with the molecular formula C15 H10 O4 by analyses and comparison. The antimicrobial activity of puri-fied Aloesaponarin II against Xoo race P6 was tested taking chloromycetin as a control. The results showed that Aloe-saponarin II possessed fairly strong antibiotic activity,its semi-inhibition concentration was IC50 = 19. 2 μg/ mL,but weaker than that of chloromycetin(IC50 = 0. 39 μg/ mL).