微生物学杂志
微生物學雜誌
미생물학잡지
JOURNAL OF MICROBIOLOGY
2014年
4期
37-41
,共5页
戚菲菲%王锐莹%刘静%曾胜%刘北星
慼菲菲%王銳瑩%劉靜%曾勝%劉北星
척비비%왕예형%류정%증성%류북성
呼吸道合胞病毒%IL-33%肺泡巨噬细胞%树突状细胞
呼吸道閤胞病毒%IL-33%肺泡巨噬細胞%樹突狀細胞
호흡도합포병독%IL-33%폐포거서세포%수돌상세포
respiratory syncytial virus(RSV)%IL-33%alveolar macrophages%dendritic cells
IL-33是新发现的细胞因子,在病毒感染所诱发的气道炎症反应中发挥重要作用。研究发现感染呼吸道合胞病毒的 BALB/ c 鼠肺组织内 IL-33水平明显升高。但 RSV 感染后分泌 IL-33的免疫细胞类型,尤其是感染早期分泌 IL-33的固有免疫细胞类型目前尚不清楚。通过分离培养 BALB/ c 鼠肺泡巨噬细胞及树突状细胞,采用 ELISA 及实时荧光定量 PCR 法检测上述固有免疫细胞 RSV 感染后 IL-33分泌水平的变化。结果显示,RSV 感染72 h 后 BALB/ c 鼠肺泡巨噬细胞 IL-33mRNA 水平明显升高,而树突状细胞 RSV 感染24 h 后 IL-33mRNA 水平开始上升,48 h 达峰值。研究证实 RSV 感染诱导 BALB/ c 鼠固有免疫细胞分泌 IL-33,进而介导感染后炎症的发生发展。
IL-33是新髮現的細胞因子,在病毒感染所誘髮的氣道炎癥反應中髮揮重要作用。研究髮現感染呼吸道閤胞病毒的 BALB/ c 鼠肺組織內 IL-33水平明顯升高。但 RSV 感染後分泌 IL-33的免疫細胞類型,尤其是感染早期分泌 IL-33的固有免疫細胞類型目前尚不清楚。通過分離培養 BALB/ c 鼠肺泡巨噬細胞及樹突狀細胞,採用 ELISA 及實時熒光定量 PCR 法檢測上述固有免疫細胞 RSV 感染後 IL-33分泌水平的變化。結果顯示,RSV 感染72 h 後 BALB/ c 鼠肺泡巨噬細胞 IL-33mRNA 水平明顯升高,而樹突狀細胞 RSV 感染24 h 後 IL-33mRNA 水平開始上升,48 h 達峰值。研究證實 RSV 感染誘導 BALB/ c 鼠固有免疫細胞分泌 IL-33,進而介導感染後炎癥的髮生髮展。
IL-33시신발현적세포인자,재병독감염소유발적기도염증반응중발휘중요작용。연구발현감염호흡도합포병독적 BALB/ c 서폐조직내 IL-33수평명현승고。단 RSV 감염후분비 IL-33적면역세포류형,우기시감염조기분비 IL-33적고유면역세포류형목전상불청초。통과분리배양 BALB/ c 서폐포거서세포급수돌상세포,채용 ELISA 급실시형광정량 PCR 법검측상술고유면역세포 RSV 감염후 IL-33분비수평적변화。결과현시,RSV 감염72 h 후 BALB/ c 서폐포거서세포 IL-33mRNA 수평명현승고,이수돌상세포 RSV 감염24 h 후 IL-33mRNA 수평개시상승,48 h 체봉치。연구증실 RSV 감염유도 BALB/ c 서고유면역세포분비 IL-33,진이개도감염후염증적발생발전。
IL-33 is a newly found cytokine;it plays an important role in virus infection induced airway inflammation reaction. It was found in the study that the level of IL-33 was significantly increased in the lungs of BALB/ c mice in-fected with respiratory syncytial virus(RSV). However,the immune cell types for IL-33 excretion after RSV infec-tion,especially the cell types of the innate immune cells,remain unknown so far. In this study,by isolating and cul-tivating alveolar macrophages and dendritic cells from BALB/ c mice,the change of IL-33 excretion level in innate im-mune cells after infected with RSV were tested and determined with ELISA and real-time PCR. The results showed that at 72 h after RSV infection,the expression of IL-33 mRNA level in the alveolar macrophages increased signifi-cantly. Meanwhile,the expression of IL-33 mRNA level in dendritic cells began to increase 24 h after the infection, and reached to a peak at 48 h after RSV infection. This study has confirmed that RSV infection induced BALB/ c mice innate immune cells to excrete IL-33,and proceed to medium-leading the occurrence and development of inflammation after the infection.