CAJ | 학술논문

酿酒酵母细胞中 ymr034c 基因与白念珠菌的 Carch1基因同源，CaRCH1与白念珠菌对钙离子、锂离子和硝唑类药物的耐受性相关。因此，把 ymr034c 命名为 Scrch1。前期研究结果显示，在胞外高钙离子胁迫条件下，ScRCH1定位于细胞质膜上。为了研究 Scrch1基因表达的调控机理，通过荧光显微镜技术对酵母细胞基因组中编码转录因子的223个单基因缺失菌株进行了筛选，分别检测了 ScRCH1-GFP 融合蛋白在它们中的亚细胞定位情况。结果发现，钙离子处理后，ngg1、hal9、crz1、ada2和 swi6五个转录因子基因的缺失造成ScRCH1-GFP 没有细胞质膜定位，而 ino2基因的缺失导致 ScRCH1-GFP 在不经钙离子处理的条件下即定位到细胞质膜上。
양주효모세포중 ymr034c 기인여백념주균적 Carch1기인동원，CaRCH1여백념주균대개리자、리리자화초서류약물적내수성상관。인차，파 ymr034c 명명위 Scrch1。전기연구결과현시，재포외고개리자협박조건하，ScRCH1정위우세포질막상。위료연구 Scrch1기인표체적조공궤리，통과형광현미경기술대효모세포기인조중편마전록인자적223개단기인결실균주진행료사선，분별검측료 ScRCH1-GFP 융합단백재타문중적아세포정위정황。결과발현，개리자처리후，ngg1、hal9、crz1、ada2화 swi6오개전록인자기인적결실조성ScRCH1-GFP 몰유세포질막정위，이 ino2기인적결실도치 ScRCH1-GFP 재불경개리자처리적조건하즉정위도세포질막상。
Gene of ymr034c in Saccharomyces cerevisiae is homologous to the gene of Carch1 in Candida albicans gene,CaRCH1 and C. albicans were related to calcium ion,lithium ion,and nidazoles resistance. Therefore,it was named ymr034c as Scrch1. The earlier study showed that ScRCH1 localizes at plasma membrane under the coercion of high levels of extracellular calcium ion. In order to study on regulation mechanism of the expression of Scrch1 gene, single-gene deletion mutants for 223 of coding transcription factor in S. cerevisiae was screened through fluorescent mi-croscopy. The results showed that after calcium ion treatment,five deletion transcription genes of ngg1,hal9,crz1, ada2,and swi6,caused ScRCH1-GFP fail to localize at the plasma membrane. In addition,the deletion of ino2 gene led ScRCH1-GFP localized to the plasma membrane under the conditions even without the treatment of calcium ion.