CAJ | 학술논문

采用平板分离和培养瓶厌氧技术，结合 PCR 扩增技术，以沙曲矿煤为发酵基底，中泰矿业和沙曲矿井水及白腐菌为菌种来源，进行产甲烷菌培养实验，并添加不同浓度梯度的 EDTA 二钠，进行产甲烷菌培养实验，测定实验结束后的产甲烷量。实验证明：中泰矿业矿井水中有产甲烷菌的存在，而沙曲矿井水在含反硝化菌和 pH 偏高的情况下，抑制了产甲烷菌的生长。同等条件下沙曲矿批次实验中没有甲烷气体的产生，而中泰矿业的有气体产出，适宜浓度的 EDTA 二钠浓度会显著提高产气量，EDTA 二钠用量为1.0 g/ L 时，中泰矿井水 CH4产率达到最大，为10.0 mL/ g 煤，反应后溶液的 pH 值明显增高。
채용평판분리화배양병염양기술，결합 PCR 확증기술，이사곡광매위발효기저，중태광업화사곡광정수급백부균위균충래원，진행산갑완균배양실험，병첨가불동농도제도적 EDTA 이납，진행산갑완균배양실험，측정실험결속후적산갑완량。실험증명：중태광업광정수중유산갑완균적존재，이사곡광정수재함반초화균화 pH 편고적정황하，억제료산갑완균적생장。동등조건하사곡광비차실험중몰유갑완기체적산생，이중태광업적유기체산출，괄의농도적 EDTA 이납농도회현저제고산기량，EDTA 이납용량위1.0 g/ L 시，중태광정수 CH4산솔체도최대，위10.0 mL/ g 매，반응후용액적 pH 치명현증고。
Adopting the plate separation technology and incubation bottles anaerobic technology,combined with the PCR amplification technology,using coal samples from Shaqu coal mine as the fermentation substrates and the mine water respectively from Zhongtai Mining and Shaqu mine and white rot fungi as the strain sources,the methane-produ-cing bacteria cultivation experiment was conducted and the methane production was determined by adding the bi-sodi-um EDTA solution with various concentration gradients. The experimental had confirmed that there exist methane-pro-ducing bacteria in the mine water of Zhongtai Mining,and under the conditions of the content of denitro-bacteria and slantly high pH in the mine water of Shaqu coal mine the growth of methane-producing bacteria was inhibited. Under equal conditions,there is no methane produced in the batch experiments coal samples from Shaqu mine,while there is a gas output in the samples of Zhongtai Mining,and appropriate concentration of bi-sodium EDTA significantly in-creased the gas production. When the bi-sodium EDTA was at 1. 0 g/ L,the CH4 production rate reached the maxi-mum at 10. 0 mL/ g,and the pH value increases notably after the reaction.