CAJ | 학술논문

在盆栽试验条件下，研究在3个遮阴水平和5个施氮水平处理组合下，水稻分蘖期、孕穗期和抽穗期冠层叶片叶绿素计测定值（soil and plant analyzer development value，SPAD value）的空间分布规律及其差异，为弱光条件下实时进行水稻叶片氮素营养诊断及优化提供依据.结果表明，同对照相比，在遮阴条件下水稻冠层 SPAD值波动幅度受到明显抑制.遮光使水稻冠层叶片 SPAD值下降，并抑制施氮水平对水稻叶片向上位叶输送氮素的正向作用.经变异系数分析发现，L4叶对施氮水平变化反应最为敏感.另外，在中度和重度遮阴条件（遮光率分别为65%和85%）下可利用叶片相对叶色差值（relative SPAD value，RSPAD）进行氮素营养状态的诊断.相对叶色差值SPADL3-L2，SPADL4-L3与施氮水平呈显著线性正相关关系（R2L3-L2=0.87~0.97，R2L4-L3=0.85~0.97），这一关系不受生育期和施氮水平的影响.在正常光照条件下，宜选用 SPADL3-L2指示水稻整个生育期氮素营养状况.在日照较弱的季节或受林木遮光影响较大的田块，宜选择 SPADL4-L3作为诊断指标.
재분재시험조건하，연구재3개차음수평화5개시담수평처리조합하，수도분얼기、잉수기화추수기관층협편협록소계측정치（soil and plant analyzer development value，SPAD value）적공간분포규률급기차이，위약광조건하실시진행수도협편담소영양진단급우화제공의거.결과표명，동대조상비，재차음조건하수도관층 SPAD치파동폭도수도명현억제.차광사수도관층협편 SPAD치하강，병억제시담수평대수도협편향상위협수송담소적정향작용.경변이계수분석발현，L4협대시담수평변화반응최위민감.령외，재중도화중도차음조건（차광솔분별위65%화85%）하가이용협편상대협색차치（relative SPAD value，RSPAD）진행담소영양상태적진단.상대협색차치SPADL3-L2，SPADL4-L3여시담수평정현저선성정상관관계（R2L3-L2=0.87~0.97，R2L4-L3=0.85~0.97），저일관계불수생육기화시담수평적영향.재정상광조조건하，의선용 SPADL3-L2지시수도정개생육기담소영양상황.재일조교약적계절혹수림목차광영향교대적전괴，의선택 SPADL4-L3작위진단지표.
Summary The application of real-time,site-specific nitrogen management can guarantee the high yield of rice, and improve the utilization rate of nitrogen.The management pointed out that soil and plant analyzer development value (SPAD value)could be an index for nitrogen application precise precision,namely using chlorophyll meter as a nitrogen nutrition diagnosis tools on rice.Previous studies had looked at the effects of cultivars,growth stage,leaf position and other factors on SPAD value,while research on the influence of light and nitrogen on rice canopy SPAD value is few in our country.Therefore,it is necessary to study the distribution characteristics of SPAD value under low light conditions.And the feasibility of applying chlorophyll meter on nitrogen nutrition diagnosis was investigated. <br> This article studied the methods to optimize nitrogen nutrition diagnosis of rice leaves under low-light stress condition with SPAD meter. <br> Pot experiments were conducted to study the distributing characteristics of canopy SPAD and the distributional difference at tillering,booting and heading stages.There were three levels of shading and five levels of nitrogen supply in the experiment.There exists a law that the greenness of rice leaf was regularly changed as black-yellow alternation ways through the whole growth period of rice despite of the effect of light deficiency.But the fluctuation was significantly suppressed under shading conditions.The decrease of SPAD value resulted from lacking light. Improving nitrogen level had positive effect on increasing the SPAD values of functional leaves.However,less-light condition made an inhibitory effect on the positive role of nitrogen.Shading treatment promoted the increment of lower leaf SPAD values.The fourth leaf(L4)has an instruction function for nitrogen nutrition status during the key growing periods under the interaction of light density and nitrogen supply,and it may be a better leaf indicator for rice N status.SPADL3-L2 and SPADL4-L3(relative SPAD value,RSPAD) were remarkably correlated linearly with nitrogen level(under full sunlight,R2L3-L2=0.87 0.97;under shading treatment,R2L4-L3=0.85 0.97)without considering the influence from the growing stages and nitrogen rates. <br> For Hang 43,SPAD values of the top four leaves on main stem could be used as an indicator for diagnosis of nitrogen status and nitrogenous fertilizer application in rice under shading. Under the natural ultraviolet radiation condition, SPADL3-L2 will be a better one for nutrition diagnosis.When light intensity is low and less (deficient sunshine or light attenuation by mutual shading during the growing period of rice),SPADL4-L3 may perform better for N status diagnosis.