CAJ | 학술논문

目的：探讨血必净注射液对中暑大鼠血管内皮细胞的保护机制。方法将90只SD大鼠按随机数字表法分为对照组、模型组、血必净治疗组，每组30只。将大鼠置于40℃、60%相对湿度的恒温箱中1 h复制中暑模型，血必净治疗组于制模后腹腔注射血必净注射液2.5 g/kg 1次，对照组和模型组腹腔注射生理盐水2 mL/kg 1次。各组于给药后2、6、12 h记录直肠温度、心率、平均动脉压（MAP）；同时取大鼠腹主动脉血分离血清，用酶联免疫吸附试验（ELISA）测定脂多糖（LPS）、核转录因子-κB（NF-κB）、p53水平，用全自动血凝分析仪测定凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、D-二聚体水平。结果与对照组比较，模型组直肠温度、心率、LPS、NF-κB、p53、PT、APTT、D-二聚体均明显升高，MAP均明显降低（P＜0.05或P＜0.01）；与模型组比较，血必净治疗组于制模后2 h上述指标即明显改善〔直肠温度（℃）：38.02±0.22比39.32±0.33，心率（次/min）：507±14比562±35，MAP（mmHg，1 mmHg=0.133 kPa）：98±6比87±13，LPS （ng/L）：0.65±0.03比0.82±0.05，NF-κB（ng/L）：1.10±0.04比1.33±0.05，p53（ng/L）：1.33±0.03比1.73±0.02， PT（s）：15.47±1.03比20.28±2.01，APTT（s）：40.26±2.46比47.46±3.51，D-二聚体（μg/L）：238.54±8.32比323.12±8.14，P＜0.05或P＜0.01〕。结论血必净注射液是通过减少中暑大鼠血管内皮细胞分泌NF-κB、p53水平，纠正血液PT、APTT、D-二聚体的紊乱而保护血管内皮细胞的完整性从而抑制LPS进入血液。
목적：탐토혈필정주사액대중서대서혈관내피세포적보호궤제。방법장90지SD대서안수궤수자표법분위대조조、모형조、혈필정치료조，매조30지。장대서치우40℃、60%상대습도적항온상중1 h복제중서모형，혈필정치료조우제모후복강주사혈필정주사액2.5 g/kg 1차，대조조화모형조복강주사생리염수2 mL/kg 1차。각조우급약후2、6、12 h기록직장온도、심솔、평균동맥압（MAP）；동시취대서복주동맥혈분리혈청，용매련면역흡부시험（ELISA）측정지다당（LPS）、핵전록인자-κB（NF-κB）、p53수평，용전자동혈응분석의측정응혈매원시간（PT）、활화부분응혈활매시간（APTT）、D-이취체수평。결과여대조조비교，모형조직장온도、심솔、LPS、NF-κB、p53、PT、APTT、D-이취체균명현승고，MAP균명현강저（P＜0.05혹P＜0.01）；여모형조비교，혈필정치료조우제모후2 h상술지표즉명현개선〔직장온도（℃）：38.02±0.22비39.32±0.33，심솔（차/min）：507±14비562±35，MAP（mmHg，1 mmHg=0.133 kPa）：98±6비87±13，LPS （ng/L）：0.65±0.03비0.82±0.05，NF-κB（ng/L）：1.10±0.04비1.33±0.05，p53（ng/L）：1.33±0.03비1.73±0.02， PT（s）：15.47±1.03비20.28±2.01，APTT（s）：40.26±2.46비47.46±3.51，D-이취체（μg/L）：238.54±8.32비323.12±8.14，P＜0.05혹P＜0.01〕。결론혈필정주사액시통과감소중서대서혈관내피세포분비NF-κB、p53수평，규정혈액PT、APTT、D-이취체적문란이보호혈관내피세포적완정성종이억제LPS진입혈액。
ObjectiveTo investigate the mechanism of protective effect of Xuebijing injection on vascular endothelial cells in rats with heat stress.Methods Ninety Sprague-Dawley(SD) rats were randomly divided into control, model and Xuebijing injection treatment groups, 30 rats in each group. Heat stress model was reproduced by placing rats in constant temperature box at 40℃, 60% relative humidity for 1 hour, Xuebijing injection group was treated by intraperitoneal injection of Xuebijing 2.5 g/kg, while the control and model groups were treated by intraperitoneal injection of normal saline 2 mL/kg, once a day only in 1 day for both groups. After model establishment, the rectum temperature, heart rate and the mean arterial pressure(MAP) were recorded at 2, 6, 12 hours in each group. At the same time, the rat abdominal aortic blood was collected and serum was separated, the enzyme-linked immunosorbent assay(ELISA) was used to determine the aortic serum levels of lipopolysaccharide(LPS), nuclear factor-κB(NF-κB) and p53, and the prothrombin time(PT), activated partial thromboplastin time(APTT) and D-dimer of venous blood were detected by automatic blood coagulation analyzer(ACLTOP).Results Compared with those in control group, the rectum temperature, heart rate, LPS, NF-κB, p53, PT, APTT, D-dimer were significantly increased, and MAP was obviously decreased in model group(P<0.05 orP<0.01). Compared with model group, the above indexes were improved significantly in Xuebijing injection treatment group at 2 hours〔rectum temperature(℃): 38.02±0.22 vs. 39.32±0.33, heart rate(bpm): 507±14 vs. 562±35, MAP(mmHg, 1 mmHg=0.133 kPa): 98±6 vs. 87±13, LPS(ng/L): 0.65±0.03 vs. 0.82±0.05, NF-κB(ng/L): 1.10±0.04 vs. 1.33±0.05, p53(ng/L): 1.33±0.03 vs. 1.73±0.02, PT(s):15.47±1.03 vs. 20.28±2.01, APTT(s): 40.26±2.46 vs. 47.46±3.51, D-dimer(μg/L): 238.54±8.32 vs. 323.12±8.14,P<0.05 orP<0.01〕.Conclusion Xuebijing injection can correct the disorders of blood PT, APTT, D-dimer via decreasing the secretion of the levels of NF-κB, p53 from vascular endothelial cells in rats with heat stress, thus the integrity of the vascular endothelium can be protected, and LPS entering into the blood stream can be inhibited.