CAJ | 학술논문

目的：观察慢性乙型肝炎病毒感染产妇新生儿脐血中树突状细胞亚群（DC ）的变化与乙肝病毒核酸（HBV DNA）载量的关系。方法采集28例慢性乙型肝炎病毒感染产妇所生新生儿脐血，用三色分析法进行免疫荧光标记，24 h内上流式细胞仪进行树突状细胞的检测。同时对慢性乙型肝炎感染的新生儿脐血分离血清，进行HBV DNA载量的检测。结果慢性HBV感染产妇和健康产妇新生儿脐血DC1、DC2和DC1/DC2比较，差异均无统计学意义；慢性HBV感染产妇新生儿脐血HBV DNA阴性（HBVDNA＜103拷贝/ml）、阳性（HBVDNA≥103拷贝/ml）分别为57.1％和42.9％；HBV DNA≥106拷贝/ml、HBV DNA＜106拷贝/ml分别为41.7％和58.3％；HBV DNA阳性者DC1、DC2低于HBV DNA阴性者，脐血HBV DNA阳性中HBV DNA＞106拷贝/ml者的DC1和DC2相对数均明显低于HBV DNA＜106拷贝/ml者，差异均有统计学意义。结论慢性乙型肝炎病毒感染产妇新生儿并未全部感染乙肝病毒；慢性乙型肝炎病毒感染产妇新生儿脐血中树突状细胞亚群相对数与脐血中HBV DNA含量有关，母血清HBV DNA含量≥106拷贝/ml时，胎盘感染乙肝病毒的危险性显著增加。
목적：관찰만성을형간염병독감염산부신생인제혈중수돌상세포아군（DC ）적변화여을간병독핵산（HBV DNA）재량적관계。방법채집28례만성을형간염병독감염산부소생신생인제혈，용삼색분석법진행면역형광표기，24 h내상류식세포의진행수돌상세포적검측。동시대만성을형간염감염적신생인제혈분리혈청，진행HBV DNA재량적검측。결과만성HBV감염산부화건강산부신생인제혈DC1、DC2화DC1/DC2비교，차이균무통계학의의；만성HBV감염산부신생인제혈HBV DNA음성（HBVDNA＜103고패/ml）、양성（HBVDNA≥103고패/ml）분별위57.1％화42.9％；HBV DNA≥106고패/ml、HBV DNA＜106고패/ml분별위41.7％화58.3％；HBV DNA양성자DC1、DC2저우HBV DNA음성자，제혈HBV DNA양성중HBV DNA＞106고패/ml자적DC1화DC2상대수균명현저우HBV DNA＜106고패/ml자，차이균유통계학의의。결론만성을형간염병독감염산부신생인병미전부감염을간병독；만성을형간염병독감염산부신생인제혈중수돌상세포아군상대수여제혈중HBV DNA함량유관，모혈청HBV DNA함량≥106고패/ml시，태반감염을간병독적위험성현저증가。
Objective Tocharacterizethevariationsofcordblooddendriticcells(DC)subsetisolatedfrom fetuses whose mothers had chronic hepatitis B (CHB),to examine the relationships between these variations and HBV load,and to elucidate the transmission mechanism of CHB from mothers to their babies. Methods We detected 28 cord blood samples from fetuses whose mothers had chronic hepatitis B for the DC subsets.All the cord blood samples were detected immediately after being collected,and the percentages of DC1 and DC2 were determined by three-color cytometry. The samples from fetuses whose mothers had chronic hepatitis B were centrifuged,and assessed for HBVDNA load. Results In all of the 28 samples of fetuses whose mothers had chronic hepatitis B,12 was positive,and the others was negative.In the samples with HBV DNA positive,we found that the percentages of DC1 and DC2 were lower in cord blood whose HBV DNA was positive than it was negative. Conclusion Not all of the newborns were infected although their mothers had chronic hepatitis B.The relative quantity of the DC1 and DC2 in cord blood whose mothers had CHB was associated with HBV DNA level.