西北药学杂志
西北藥學雜誌
서북약학잡지
2014年
5期
446-449
,共4页
崔誉文%杨黎彬%杨静%许苗苗%程茜菲%宋小妹%崔九成
崔譽文%楊黎彬%楊靜%許苗苗%程茜菲%宋小妹%崔九成
최예문%양려빈%양정%허묘묘%정천비%송소매%최구성
黄翘%连翘苷%连翘酯苷A%表松脂素%HPLC%指纹图谱
黃翹%連翹苷%連翹酯苷A%錶鬆脂素%HPLC%指紋圖譜
황교%련교감%련교지감A%표송지소%HPLC%지문도보
Fructus f orsythiae%forsythin%forsythoside A%pinoresinol%HPLC%fingerprint
目的:建立黄翘 HPLC指纹图谱,为黄翘的质量控制提供理论依据。方法色谱柱:Thermo C18 Column(250 mm ×4.6 mm ,5μm);流动相:乙腈-水,梯度洗脱;检测波长:203 nm。结果生成了黄翘的对照指纹图谱,标定了17个共有峰,并确定连翘苷、连翘酯苷A和表松脂素各峰的归属。结论黄翘的 HPLC指纹图谱稳定,重复性良好,信息涵盖量大,为进一步科学评价黄翘的质量提供依据。
目的:建立黃翹 HPLC指紋圖譜,為黃翹的質量控製提供理論依據。方法色譜柱:Thermo C18 Column(250 mm ×4.6 mm ,5μm);流動相:乙腈-水,梯度洗脫;檢測波長:203 nm。結果生成瞭黃翹的對照指紋圖譜,標定瞭17箇共有峰,併確定連翹苷、連翹酯苷A和錶鬆脂素各峰的歸屬。結論黃翹的 HPLC指紋圖譜穩定,重複性良好,信息涵蓋量大,為進一步科學評價黃翹的質量提供依據。
목적:건립황교 HPLC지문도보,위황교적질량공제제공이론의거。방법색보주:Thermo C18 Column(250 mm ×4.6 mm ,5μm);류동상:을정-수,제도세탈;검측파장:203 nm。결과생성료황교적대조지문도보,표정료17개공유봉,병학정련교감、련교지감A화표송지소각봉적귀속。결론황교적 HPLC지문도보은정,중복성량호,신식함개량대,위진일보과학평개황교적질량제공의거。
Objective To establish an HPLC fingerprint of Fructus forsythiae .Methods The chromatographic separation was per-formed on a C18 column (250 mm × 4 .6 mm ,5μm) .The mobile phase was a mixture of acetonitrile-water solution in gradient elu-tion .The flow rate was 1 .0 mL · min-1 ,and the detection wavelength was 203 nm .Results The HPLC fingerprint of plantaginis semen showed 17 characteristic peaks with high similarity ,in which three peaks were identified .Conclusion The established method is simple ,accurate and reproduceable ,and can be used for the quality control of Fructus forsythiae samples .