CAJ | 학술논문

目的：建立丹参中隐丹参酮和丹参酮Ⅱ A 的含量测定方法。方法采用微乳液相色谱法。色谱柱：Diamonsal C18（250 mm ×4．6 mm ，5μm）；流动相：0．14 mol · L -1十二烷基硫酸钠-1．08 mol · L -1正丁醇-0．070 mol · L -1正辛烷-水微乳；流速：0．7 mL · min-1；柱温：室温；检测波长：270 nm。结果隐丹参酮和丹参酮ⅡA分别在0．962～38．48和1．022～40．88μg范围内线性关系良好，相关系数 r＞0．9995；平均加样回收率（n＝6）分别为96．5％和97．2％，RSD分别为2．71％和1．69％。结论该方法准确，简单易行，可用于丹参药材中2种有效成分的含量分析。
목적：건립단삼중은단삼동화단삼동Ⅱ A 적함량측정방법。방법채용미유액상색보법。색보주：Diamonsal C18（250 mm ×4．6 mm ，5μm）；류동상：0．14 mol · L -1십이완기류산납-1．08 mol · L -1정정순-0．070 mol · L -1정신완-수미유；류속：0．7 mL · min-1；주온：실온；검측파장：270 nm。결과은단삼동화단삼동ⅡA분별재0．962～38．48화1．022～40．88μg범위내선성관계량호，상관계수 r＞0．9995；평균가양회수솔（n＝6）분별위96．5％화97．2％，RSD분별위2．71％화1．69％。결론해방법준학，간단역행，가용우단삼약재중2충유효성분적함량분석。
Objective To develop an MELC method for determining cryptotanshinone and tanshinone in Salviamiltiorrhiza simulta-neously .Methods A Diamonsil C18 column (250 mm × 4 .6 mm ,5 μm) was used ,and the mobile phase consisted of 0 .14 mol · L-1 SDS-1 .08 mol · L -1 n-butanol-0 .070 mol · L -1 n-octane-water at a flow rate of 0 .7 mL · min-1 .The column temperature was room temperature ,and the detection wavelength was set at 270 nm .Results The linear range of cryptotanshinone and tanshinoneⅡA were within 0 .962-38 .48 and 1 .022-40 .88 μg ,respectively .The correlation coefficient r were 0 .999 5 and 0 .999 8 ,respec-tively .The average recoveries (n=6) were 96 .5% with RSD=2 .71% (n=6) and 97 .2% with RSD=1 .69% (n=6) .Conclu-sion This method was simple ,accurate and reliable ,and could be applied in the determination of the two components in Salvia miltiorrhiz a .