浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2014年
9期
897-900
,共4页
何婷婷%陈金%张建华%蒋卓婧%胡雪娜%傅利军%钦博
何婷婷%陳金%張建華%蔣卓婧%鬍雪娜%傅利軍%欽博
하정정%진금%장건화%장탁청%호설나%부리군%흠박
手足口病%肠道病毒71 型%基因型%同源性
手足口病%腸道病毒71 型%基因型%同源性
수족구병%장도병독71 형%기인형%동원성
Hand-foot-and-mouth disease%Enterovirus type 7 1%Genotypes%Homogeneity
目的：研究绍兴市2012-2013年手足口病患者的肠道病毒71型（EV 71）病毒分离株的基因特征。方法采集手足口病患者粪便、疱疹液和咽拭子标本，采用逆转录聚合酶链反应（RT-PCR）特异性扩增进行EV 71鉴定，阳性的样本进行病毒分离，对10株分离株分A、B、C、D和E 五段进行分段PCR，测序后并拼接成全长，以EV 71 A、B和C各基因型的参考毒株和以往中国EV 71的分离毒株为参考株，基于核苷酸序列和氨基酸序列对VP1区分别进行同源性分析和系统发生树构建。结果 RT-PCR结果证实分离的10株病毒均为EV 71，与C4a亚型亲缘关系最近，它们之间核苷酸和氨基酸同源性分别为95.06％～97.87％和93.60％～98.99％；与亲缘关系最远的参考毒株Cox A16的核苷酸和氨基酸同源性分别为61.17％～62.74％和68.00％～71.38％。在系统发生树上，这10株EV 71均在C基因型中，与属C4a亚型的病毒株属同一分支。结论绍兴地区手足口病患者中分离的EV 71毒株属C基因型的C4a亚型进化分支。
目的：研究紹興市2012-2013年手足口病患者的腸道病毒71型（EV 71）病毒分離株的基因特徵。方法採集手足口病患者糞便、皰疹液和嚥拭子標本，採用逆轉錄聚閤酶鏈反應（RT-PCR）特異性擴增進行EV 71鑒定，暘性的樣本進行病毒分離，對10株分離株分A、B、C、D和E 五段進行分段PCR，測序後併拼接成全長，以EV 71 A、B和C各基因型的參攷毒株和以往中國EV 71的分離毒株為參攷株，基于覈苷痠序列和氨基痠序列對VP1區分彆進行同源性分析和繫統髮生樹構建。結果 RT-PCR結果證實分離的10株病毒均為EV 71，與C4a亞型親緣關繫最近，它們之間覈苷痠和氨基痠同源性分彆為95.06％～97.87％和93.60％～98.99％；與親緣關繫最遠的參攷毒株Cox A16的覈苷痠和氨基痠同源性分彆為61.17％～62.74％和68.00％～71.38％。在繫統髮生樹上，這10株EV 71均在C基因型中，與屬C4a亞型的病毒株屬同一分支。結論紹興地區手足口病患者中分離的EV 71毒株屬C基因型的C4a亞型進化分支。
목적：연구소흥시2012-2013년수족구병환자적장도병독71형（EV 71）병독분리주적기인특정。방법채집수족구병환자분편、포진액화인식자표본，채용역전록취합매련반응（RT-PCR）특이성확증진행EV 71감정，양성적양본진행병독분리，대10주분리주분A、B、C、D화E 오단진행분단PCR，측서후병병접성전장，이EV 71 A、B화C각기인형적삼고독주화이왕중국EV 71적분리독주위삼고주，기우핵감산서렬화안기산서렬대VP1구분별진행동원성분석화계통발생수구건。결과 RT-PCR결과증실분리적10주병독균위EV 71，여C4a아형친연관계최근，타문지간핵감산화안기산동원성분별위95.06％～97.87％화93.60％～98.99％；여친연관계최원적삼고독주Cox A16적핵감산화안기산동원성분별위61.17％～62.74％화68.00％～71.38％。재계통발생수상，저10주EV 71균재C기인형중，여속C4a아형적병독주속동일분지。결론소흥지구수족구병환자중분리적EV 71독주속C기인형적C4a아형진화분지。
Objective To undersyand the genetic characteristics of Enterovirus Type 71 (EV 71 )isolated from clinical specimens of children with hand-foot-mouth disease (HFMD)in Shaoxing city from 2012 to 2013.Methods RNA was extracted from specimens collected from stool,throat swab and vesicle from children with HFMD,and EV 71 were identified by reverse transcriptase polymerase chain reaction (RT-PCR).Ten positive samples were isolated and were amplified by segmented PCR,then,they were spliced into a full-length after sequenced according to the reference strains. Homology analysis on region VP1 and phylogenetic tree were carried out based on nucleotide sequence and amino acid sequence.Results The results indicated that the ten isolated strains were all EV 71 positive.Sequence alignment of VP1 showed that the nucleotide and amino acid homogeneity of these 10 strains were related to C4a genotypes,with 95.06%-97.87% and 93.60%-98.99% respectively.Phylogenetic tree based on VP1 indicated that these 10 strains all belonged to C4a genotype.Conclusion The identified EV 71 strains isolated from HFMD children in Shaoxing city belong to genotype C4a.
