浙江预防医学
浙江預防醫學
절강예방의학
ZHEJIANG JOURNAL OF PREVENTIVE MEDICINE
2014年
9期
880-883
,共4页
姚亚萍%张佳峰%徐云%潘晓红%杨介者%郭志宏%夏燕%黄学敏
姚亞萍%張佳峰%徐雲%潘曉紅%楊介者%郭誌宏%夏燕%黃學敏
요아평%장가봉%서운%반효홍%양개자%곽지굉%하연%황학민
HIV-1%男男性行为%基因特征
HIV-1%男男性行為%基因特徵
HIV-1%남남성행위%기인특정
HIV-1 strains%Men who have sex with men (MSM)%Genetic characteristics
目的：分析浙江省2004-2011年117名男男性接触者（MSM）HIV-1感染病毒的序列特征，了解HIV-1毒株类型和特征。方法从实验室留样的MSM HIV感染者血液中提取DNA或RNA，用巢式PCR或RT-PCR方法扩增gag、pol基因区片段，测定序列并分析。结果117名MSM HIV感染者中，序列结果按户籍来源覆盖21个省（市、自治区），感染毒株包括CRF01_AE 99名（84.62％）、B亚型7名（5.98％）、CRF07_BC 6名（5.13％）、CRF08_BC和CRF59_01B各1名（0.85％）。3名（2.56％）疑似01_B重组毒株与安徽感染者在系统进化树上聚集成可靠的次级进化簇（99％），且具有类似重组断点模式。84个CRF01_AE毒株的pol区基因序列聚集成簇，形成多个可靠的次级进化簇（30个节点的bootstrap值高于70％）。结论浙江省MSM HIV感染者主要流行CRF01_AE毒株，该人群HIV感染率高，应加强监测。CRF59_01B和新的01B重组毒株在浙江首次检出。
目的：分析浙江省2004-2011年117名男男性接觸者（MSM）HIV-1感染病毒的序列特徵，瞭解HIV-1毒株類型和特徵。方法從實驗室留樣的MSM HIV感染者血液中提取DNA或RNA，用巢式PCR或RT-PCR方法擴增gag、pol基因區片段，測定序列併分析。結果117名MSM HIV感染者中，序列結果按戶籍來源覆蓋21箇省（市、自治區），感染毒株包括CRF01_AE 99名（84.62％）、B亞型7名（5.98％）、CRF07_BC 6名（5.13％）、CRF08_BC和CRF59_01B各1名（0.85％）。3名（2.56％）疑似01_B重組毒株與安徽感染者在繫統進化樹上聚集成可靠的次級進化簇（99％），且具有類似重組斷點模式。84箇CRF01_AE毒株的pol區基因序列聚集成簇，形成多箇可靠的次級進化簇（30箇節點的bootstrap值高于70％）。結論浙江省MSM HIV感染者主要流行CRF01_AE毒株，該人群HIV感染率高，應加彊鑑測。CRF59_01B和新的01B重組毒株在浙江首次檢齣。
목적：분석절강성2004-2011년117명남남성접촉자（MSM）HIV-1감염병독적서렬특정，료해HIV-1독주류형화특정。방법종실험실류양적MSM HIV감염자혈액중제취DNA혹RNA，용소식PCR혹RT-PCR방법확증gag、pol기인구편단，측정서렬병분석。결과117명MSM HIV감염자중，서렬결과안호적래원복개21개성（시、자치구），감염독주포괄CRF01_AE 99명（84.62％）、B아형7명（5.98％）、CRF07_BC 6명（5.13％）、CRF08_BC화CRF59_01B각1명（0.85％）。3명（2.56％）의사01_B중조독주여안휘감염자재계통진화수상취집성가고적차급진화족（99％），차구유유사중조단점모식。84개CRF01_AE독주적pol구기인서렬취집성족，형성다개가고적차급진화족（30개절점적bootstrap치고우70％）。결론절강성MSM HIV감염자주요류행CRF01_AE독주，해인군HIV감염솔고，응가강감측。CRF59_01B화신적01B중조독주재절강수차검출。
Objective To analyze the subtypes and characteristics of HIV-1 among men who have sex with men (MSM) in Zhejiang province from the year 2004 to 2011.Methods Blood DNA/RNA was extracted from the MSM HIV -1 infected individuals and then HIV-1 gag and pol fragments were amplified by nested polymerase chain reaction (nested-PCR)or RT-PCR.The positive PCR products were sequenced and the obtained sequences were analyzed by phylogenetic inference.Results A total of 117 HIV-1 infected MSMindividuals were analyzed,covering 21 provinces according to their household registry.Totally,three major strains were identified including CRF01_AE (84.62%,n =99 ),B (5.98%,n=7)and CRF07_BC (5.13%,n=6). Moreover,3 novel 01B recombinant stains,1 CRF59_01B and 1 CRF08_BC were found.According to the neighbor-joining tree by pol fragments,there were more than 30 sub clusters with bootstrap value higher than 70% among the 84 CRF01_AE sequences.Conclusion CRF01_AE is the major HIV-1 infection strain among MSMin Zhejiang province.CRF59_01B and other novel 01B recombinant stains are first reported among MSM individuals in Zhejiang province.
