CAJ | 학술논문

目的：设计膝关节打击器，并评估其制作兔膝关节软骨下骨微损伤模型的稳定性。方法遵循重物坠落致伤原理设计膝关节打击器。将12只新西兰大白兔随机分成两组，A组6只兔6个膝关节为实验组，对侧膝关节为对照组，将质量1.74 kg重物从30 cm高自由坠落，撞击髌股关节。B组6只兔6个膝关节为对象，对侧膝关节为对照组，将质量1.14 kg重物从28 cm高自由坠落，撞击兔股骨外侧髁。分别于撞击前后行X线检查，撞击后行MRI检查，取股骨髁部行HE染色。结果 X线平片检测，股骨均未出现骨折。A组MRI检查发现6个膝关节股骨干骺端T1加权像、T2加权像和脂肪抑制像低信号；HE染色显示软骨和软骨下骨皮质完整，骨小梁局部排列紊乱或连续性中断，髓腔内可见多处不规则排列大片红细胞。B组1只兔膝关节MRI图像质量较差，余5个膝关节可见股骨外侧髁处T1加权像低信号，T2加权像和脂肪抑制像高信号；HE染色显示软骨和软骨下骨皮质完整，骨小梁局部排列紊乱。对照组MRI检查未见异常信号，HE染色未见异常。结论设计的膝关节打击器操作方便可控，能可靠地复制兔膝关节软骨下骨微损伤模型。
목적：설계슬관절타격기，병평고기제작토슬관절연골하골미손상모형적은정성。방법준순중물추락치상원리설계슬관절타격기。장12지신서란대백토수궤분성량조，A조6지토6개슬관절위실험조，대측슬관절위대조조，장질량1.74 kg중물종30 cm고자유추락，당격빈고관절。B조6지토6개슬관절위대상，대측슬관절위대조조，장질량1.14 kg중물종28 cm고자유추락，당격토고골외측과。분별우당격전후행X선검사，당격후행MRI검사，취고골과부행HE염색。결과 X선평편검측，고골균미출현골절。A조MRI검사발현6개슬관절고골간후단T1가권상、T2가권상화지방억제상저신호；HE염색현시연골화연골하골피질완정，골소량국부배렬문란혹련속성중단，수강내가견다처불규칙배렬대편홍세포。B조1지토슬관절MRI도상질량교차，여5개슬관절가견고골외측과처T1가권상저신호，T2가권상화지방억제상고신호；HE염색현시연골화연골하골피질완정，골소량국부배렬문란。대조조MRI검사미견이상신호，HE염색미견이상。결론설계적슬관절타격기조작방편가공，능가고지복제토슬관절연골하골미손상모형。
Objective To develop a knee joint impact device to produce animal model of subchondral bone micro-fracture in rabbits. Methods A knee joint impact device was designed. 12 New Zealand rabbits were divided into group A (n=6) and Group B (n=6). In the group A, 6 patellar-femoral joints of the 6 rabbits were impacted (1.74 kg from height of 30 cm). In the group B, 6 femoral condyles of the 6 rabbits were impacted (1.14 kg from height of 28 cm). All the rabbits were evaluated with X-ray and MRI post injury. The femora of all the rabbits were observed with HE staining. Results No fracture line was found in all the animals with X-rays. The MRI of the 6 knee joints in the group A showed low signal intensities on T1 weighted, T2 weighted and fat suppression images. Histopathology demonstrated integrated cartilage and sunchondral bone plate, micro-fractures of cancellous and bleeding in marrow. The MRI of the 5 knee joints in the group B showed low signal intensities on T1 weighted images and high signal intensities on T2 weighted and fat suppression images. Histopathology demonstrated integrated cartilage and sunchondral bone plate, micro-fractures of cancellous and bleeding in marrow. No disorder was found with MRI and histology in the contral sides. Conclusion The impact device of the knee joint can produce controllable and reproducible sub-chondral bone micro-fracture in rabbits.