中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2014年
9期
835-838
,共4页
郭知学%李鸥%汪春%冯晓梅
郭知學%李鷗%汪春%馮曉梅
곽지학%리구%왕춘%풍효매
脑外伤%胞二磷胆碱%穴位注射%足三里%神经功能%生长相关蛋白43%大鼠
腦外傷%胞二燐膽堿%穴位註射%足三裏%神經功能%生長相關蛋白43%大鼠
뇌외상%포이린담감%혈위주사%족삼리%신경공능%생장상관단백43%대서
traumatic brain injury%citicoline%acupoint injection%Zusanli (ST36)%neural function%growth associated protein-43%rats
目的：观察足三里穴位注射胞二磷胆碱疗法对脑外伤大鼠神经功能及生长相关蛋白(GAP-43)表达的影响。方法成年健康雄性Sprague-Dawley大鼠40只，除假手术组大鼠8只(A组)外，其余大鼠采用改良Feeney法建立大鼠脑损伤模型，造模成功大鼠分为穴位注药组(B组)、穴位注水组(C组)、腹腔给药组(D组)和腹腔注水组(E组)各8只，造模后每日分别给予足三里穴位注射胞二磷胆碱或生理盐水，腹腔注射胞二磷胆碱或生理盐水，连续14 d。于伤前及伤后第8、14、15、22天分别行神经功能缺损评分及旷场实验，28 d后采用免疫组化方法检测大鼠脑组织GAP-43水平。结果 B组大鼠神经缺损评分改善和旷场试验评分均优于C、D、E组(P<0.05)。B组大鼠脑组织GAP-43阳性表达多于C、D、E组(P<0.05)。结论足三里穴位注射胞二磷胆碱可促进脑外伤大鼠GAP-43蛋白表达，促进神经功能改善。
目的：觀察足三裏穴位註射胞二燐膽堿療法對腦外傷大鼠神經功能及生長相關蛋白(GAP-43)錶達的影響。方法成年健康雄性Sprague-Dawley大鼠40隻，除假手術組大鼠8隻(A組)外，其餘大鼠採用改良Feeney法建立大鼠腦損傷模型，造模成功大鼠分為穴位註藥組(B組)、穴位註水組(C組)、腹腔給藥組(D組)和腹腔註水組(E組)各8隻，造模後每日分彆給予足三裏穴位註射胞二燐膽堿或生理鹽水，腹腔註射胞二燐膽堿或生理鹽水，連續14 d。于傷前及傷後第8、14、15、22天分彆行神經功能缺損評分及曠場實驗，28 d後採用免疫組化方法檢測大鼠腦組織GAP-43水平。結果 B組大鼠神經缺損評分改善和曠場試驗評分均優于C、D、E組(P<0.05)。B組大鼠腦組織GAP-43暘性錶達多于C、D、E組(P<0.05)。結論足三裏穴位註射胞二燐膽堿可促進腦外傷大鼠GAP-43蛋白錶達，促進神經功能改善。
목적：관찰족삼리혈위주사포이린담감요법대뇌외상대서신경공능급생장상관단백(GAP-43)표체적영향。방법성년건강웅성Sprague-Dawley대서40지，제가수술조대서8지(A조)외，기여대서채용개량Feeney법건립대서뇌손상모형，조모성공대서분위혈위주약조(B조)、혈위주수조(C조)、복강급약조(D조)화복강주수조(E조)각8지，조모후매일분별급여족삼리혈위주사포이린담감혹생리염수，복강주사포이린담감혹생리염수，련속14 d。우상전급상후제8、14、15、22천분별행신경공능결손평분급광장실험，28 d후채용면역조화방법검측대서뇌조직GAP-43수평。결과 B조대서신경결손평분개선화광장시험평분균우우C、D、E조(P<0.05)。B조대서뇌조직GAP-43양성표체다우C、D、E조(P<0.05)。결론족삼리혈위주사포이린담감가촉진뇌외상대서GAP-43단백표체，촉진신경공능개선。
Objective To investigate the effects of injection of citicoline into Zusanli (ST36) acupoint on neural function and expression of growth associated protein-43 (GAP-43) in rats with traumatic brain injury. Methods 40 healthy adult male Sprague-Dawley rats were ran-domly divided into 5 groups:sham-operated group (group A, n=8), acupoint injection of citicoline group (group B, n=8), acupoint injection of saline group (group C, n=8), intraperitoneal injection of citicoline group (group D, n=8) and intraperitoneal injection of saline group (group E, n=8). Opened brain trauma was induced with the modified Feeney method in the groups B, C, D and E, and were treated as de-sign, once a day for 14 days. They were assessed with nervous function score and open-field test before and 8, 14, 15, and 22 days after inju-ry. The expression of GAP-43 in the brain were detected with immunohistochemistry 28 days after injury. Results The nervous function scores and open-field test scores improved more significantly in the group B than in the groups C, D and E (P<0.05). The expression of GAP-43 increased in the group B than in the groups C, D and E (P<0.05). Conclusion Acupoint injection of citicoline into Zusanli may im-prove the expressions of GAP-43 to promote the recovery of neural function in rats after traumatic brain injury.
