CAJ | 학술논문

通过对提取溶剂、流动相条件、最大吸收波长及色谱条件的优化，建立了一种同时测定葡萄酒中坚牢红、偶氮玉红、孟加拉红、奇通红S、荧光桃红5种酸性红色素的高效液相色谱分析方法。样品经甲醇提取后，采用Symmetry C18柱进行分离，以甲醇-20 mmol/L醋酸铵为流动相梯度洗脱，二极管阵列检测器检测，外标法定量。结果表明，各组分在0．5～50．0 mg/L范围内线性关系良好，相关系数均大于0．999，检出限为0．1～1．0 mg/kg；在1、10、50 mg/kg三个加标水平下的平均回收率为85％～106％，相对标准偏差（ RSD）为1．5％～5．8％。
통과대제취용제、류동상조건、최대흡수파장급색보조건적우화，건립료일충동시측정포도주중견뢰홍、우담옥홍、맹가랍홍、기통홍S、형광도홍5충산성홍색소적고효액상색보분석방법。양품경갑순제취후，채용Symmetry C18주진행분리，이갑순-20 mmol/L작산안위류동상제도세탈，이겁관진렬검측기검측，외표법정량。결과표명，각조분재0．5～50．0 mg/L범위내선성관계량호，상관계수균대우0．999，검출한위0．1～1．0 mg/kg；재1、10、50 mg/kg삼개가표수평하적평균회수솔위85％～106％，상대표준편차（ RSD）위1．5％～5．8％。
A high performance liquid chromatographic ( HPLC) method was developed for simultaneously detecting 5 acid red pigments such as fast red , azorubine , rose bengal , kiton red S and phloxine B in grape wine .The extracting solvent , mobile phase type , maximum absorption wavelength and chromatographic condi-tions were optimized through experiment .The sample was extracted with methanol , and the extract was separa-ted through a Symmetry C18 column (25 cm ×4.6 mm, 5μm) using methanol-20 mmol/L ammonium ace-tate as mobile phase by gradient elution .The target compounds were confirmed by diode -array detection and quantified by external standard method .The calibration curves of 5 pigments showed good linearity in the range of 0.5~50.0 mg/L with the correlative coefficients more than 0.999.The detection limits were in the range of 0.1~1.0 mg/kg.The mean recovery at 3 spiked levels of 1, 10, 50 mg/kg ranged from 85% to 106%with the RSD as 1 .5%~5 .8%.