CAJ | 학술논문

目的：比较竹叶柴胡与北柴胡抗炎、保肝作用的差异。方法采用二甲苯致耳肿胀小鼠模型及蛋清致足肿胀大鼠模型比较两种柴胡水煎液的抗炎作用；采用腹腔注射含0.1%四氯化碳( CCl4)的花生油溶液复制小鼠急性肝损伤模型，检测各组小鼠血清丙氨酸氨基转移酶( ALT)、天冬氨酸氨基转移酶( AST)、碱性磷酸酶( ALP)，肝组织匀浆超氧化物歧化酶( SOD)、丙二醛( MDA)、谷胱甘肽( GSH)；观察肝组织病理学变化及转化生长因子β( TGF-β)、核因子-κB ( NF-κB)变化。结果耳肿胀实验表明竹叶柴胡和北柴胡的水煎液均具有明显的抗炎作用，且两者差异无统计学意义(P>0.05)；竹叶柴胡组和北柴胡组ALT、AST、SOD、GSH及MDA均较模型对照组改善(P<0.05或P<0.01)，且两组改善情况相近；各治疗组ALP与模型对照组比较均差异无统计学意义(均P>0.05)。病理切片及免疫组化结果显示，竹叶柴胡组和北柴胡组TGF-β和NF-κB表达相近。结论竹叶柴胡和北柴胡的抗炎、保肝作用相近。
목적：비교죽협시호여북시호항염、보간작용적차이。방법채용이갑분치이종창소서모형급단청치족종창대서모형비교량충시호수전액적항염작용；채용복강주사함0.1%사록화탄( CCl4)적화생유용액복제소서급성간손상모형，검측각조소서혈청병안산안기전이매( ALT)、천동안산안기전이매( AST)、감성린산매( ALP)，간조직균장초양화물기화매( SOD)、병이철( MDA)、곡광감태( GSH)；관찰간조직병이학변화급전화생장인자β( TGF-β)、핵인자-κB ( NF-κB)변화。결과이종창실험표명죽협시호화북시호적수전액균구유명현적항염작용，차량자차이무통계학의의(P>0.05)；죽협시호조화북시호조ALT、AST、SOD、GSH급MDA균교모형대조조개선(P<0.05혹P<0.01)，차량조개선정황상근；각치료조ALP여모형대조조비교균차이무통계학의의(균P>0.05)。병리절편급면역조화결과현시，죽협시호조화북시호조TGF-β화NF-κB표체상근。결론죽협시호화북시호적항염、보간작용상근。
Objective To compare anti-inflammatory and hepatoprotective effects of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. Methods The anti-inflammatory effect of two kinds of radix bupleuri decoction were compared using the xylene-induced ear edema mice model and the egg white-induced foot swelling rats model. Acute liver injury in mice was induced through intraperitoneal injection of peanut oil with 0. 1%carbon tetrachloride. Alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , and alkaline phosphatase ( ALP ) in serum, superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) , and glutathione ( GSH ) in liver tissue homogenate were detected. Histopathological changes, transforming growth factor-β ( TGF-β) and nuclear factor-κB ( NF-κB) in hepatic tissues were also observed. Results Ear swelling experiment showed that the decoction of both Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. had equal anti-inflammatory effects (P>0. 05). Similarly,the decoction of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. improved levels of ALT,AST,SOD,GSH,and MDA to the same extent compared with the model control group ( P<0. 05 or P<0. 01 ). There was no significant difference among all groups in ALP levels ( P > 0. 05 ). Histopathological and immunohistochemical experiments showed that expressions of TGF-β and NF- κB were similar between the two kinds of radix bupleuri. Conclusion Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. have similar anti-inflammatory and hepatoprotective effects.